ND2 Polyclonal antibody

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  WB analysis using 19704-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19704-1-AP (ND2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 19704-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 19704-1-AP (ND2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 19704-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 19704-1-AP (ND2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

ND2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 19704-1-AP

Publications(37)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Synonyms

MT-ND2, ND 2, NADH2, NADH dehydrogenase subunit 2, MTND2

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Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IHC detected in: human stomach tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 37 publications below
• IHC: See 1 publications below

Product Information

19704-1-AP targets ND2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: NADH dehydrogenase, subunit 2 (complex I)
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 42-44 kDa
• GenBank Accession Number: GQ398480
• Gene Symbol: ND2
• Gene ID (NCBI): 4536
• RRID: AB_10638920
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P03891
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ND2, also named as MTND2, NADH2 and ND2, belongs to the complex I subunit 2 family. ND2 is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Med; The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity.; Authors - Wenzhang Wang
human	WB	Mol Cell; Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis; Authors - Yi Fu
human	WB	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang ; KD Validated
human	WB	Nat Commun; CO2-sensitive tRNA modification associated with human mitochondrial disease.; Authors - Huan Lin
human	WB	Nat Chem Biol; NSUN3 methylase initiates 5-formylcytidine biogenesis in human mitochondrial tRNA(Met).; Authors - Saori Nakano
human	WB	Nucleic Acids Res; Hypertension-associated mitochondrial DNA 4401A>G mutation caused the aberrant processing of tRNAMet, all 8 tRNAs and ND6 mRNA in the light-strand transcript.; Authors - Xiaoxu Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Morgane (Verified Customer) (10-09-2025)

Good but a little faint, could use a more lower dilution

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HCT116

ND2 Antibody Western Blot validation (1/1000 dilution) in HCT116 (Cat no:19704-1-AP)

FH

Mi (Verified Customer) (02-01-2023)

works very well in human adipocyte cell lines

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: adipocytes