ND2 Polyclonal antibody

• Featured Product
• KD/KO Validated

ND2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

25

Reviews (1)

Cat no : 19704-1-AP

Synonyms

MT ND2, MTND2, MT-ND2, NADH dehydrogenase subunit 2, NADH2, ND2

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Validation Data Gallery

• 

  WB analysis using 19704-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19704-1-AP (ND2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 25 publications below
• IHC: See 1 publications below

Product Information

19704-1-AP targets ND2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: NADH dehydrogenase, subunit 2 (complex I)
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 42-44 kDa
• GenBank Accession Number: GQ398480
• Gene Symbol: ND2
• Gene ID (NCBI): 4536
• RRID: AB_10638920
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ND2, also named as MTND2, NADH2 and ND2, belongs to the complex I subunit 2 family. ND2 is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Publications

Species	Application	Title
human	WB	Nat Med; The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity.; Authors - Wenzhang Wang
human	WB	Mol Cell; Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis; Authors - Yi Fu
human	WB	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang ; KD Validated
human	WB	Nat Commun; CO2-sensitive tRNA modification associated with human mitochondrial disease.; Authors - Huan Lin
human	WB	Nat Chem Biol; NSUN3 methylase initiates 5-formylcytidine biogenesis in human mitochondrial tRNA(Met).; Authors - Saori Nakano
human	WB	Nucleic Acids Res; Hypertension-associated mitochondrial DNA 4401A>G mutation caused the aberrant processing of tRNAMet, all 8 tRNAs and ND6 mRNA in the light-strand transcript.; Authors - Xiaoxu Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Mi (Verified Customer) (02-01-2023)

works very well in human adipocyte cell lines

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: adipocytes

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