|Positive WB detected in||A2780 cells, HEK-293 cells, mouse liver tissue, rat liver tissue, HeLa cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human breast cancer tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10233-1-AP targets NDE1 in WB, IP, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mosue, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NDE1 fusion protein Ag0336|
|Full Name||nudE nuclear distribution gene E homolog 1 (A. nidulans)|
|Calculated molecular weight||335 aa, 38 kDa|
|Observed molecular weight||40 kDa|
|GenBank accession number||BC001421|
|Gene ID (NCBI)||54820|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
NDE1 (NUDE /NUDE1) is a LIS1-interacting protein. A signaling pathway containing NDE1, LIS1, and cytoplasmic dynein is conserved for eukaryotic nuclear migration and has profound impact on neuronal migration during development of the central nervous system. LIS1 and dynein have been shown to be involved in mitosis and NDE1 participates in a subset of dynein function in M phase. NDE1 is specifically phosphorylated in M phase in its serine/threonine phosphorylation motifs, probably by Cdc2 and also Erk1 and Erk2. A fraction of NDEl binds to centrosomes strongly in interphase and is localized to mitotic spindles in early M phase. Phosphorylation of NDE1 also regulates the cell-cycle-dependent distribution, possibly by increasing its dissociation rate at the microtubule-organizing center. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human NDE1.
|Product Specific Protocols|
|WB protocol for NDE1 antibody 10233-1-AP||Download protocol|
|IHC protocol for NDE1 antibody 10233-1-AP||Download protocol|
|IF protocol for NDE1 antibody 10233-1-AP||Download protocol|
|IP protocol for NDE1 antibody 10233-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Philip (Verified Customer) (09-04-2019)
Cells fixed in PTEMF for 10min at RT. Blocked in PBS+3% BSA for 30min at RT. Primary at 1/500 for 1hr at RT.
Aurelie (Verified Customer) (09-01-2019)
lysates (15µg) from human primary Fibroblasts were loaded in 8% SDS-PAGE and immunoblotted using anti-Nde1 ( 1/1500) human primary Fibroblasts were fixed in PFA 4% and stained using anti-Nde1 ( 1/100) over-night at 4°C followed by 1h at RT with a secondary anti-rabbit Alexa (594nM) .