Tested Applications
| Positive WB detected in | A2780 cells, HEK-293 cells, HeLa cells, HepG2 cells, mouse ovary tissue, mouse brain tissue, rat brain tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human breast cancer tissue |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10233-1-AP targets NDE1 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0336 Product name: Recombinant human NDE1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 41-282 aa of BC001421 Sequence: EGSREYEAELETQLQQIETRNRDLLSENNRLRMELETIKEKFEVQHSEGYRQISALEDDLAQTKAIKDQLQKYIRELEQANDDLERAKRATIMSLEDFEQRLNQAIERNAFLESELDEKENLLESVQRLKDEARDLRQELAVQQKQEKPRTPMPSSVEAERTDTAVQATGSVPSTPIAHRGPSSSLNTPGSFRRGLDDSTGGTPLTPAARISALNIVGDLLRKVGALESKLASCRNLVYDQS Predict reactive species |
| Full Name | nudE nuclear distribution gene E homolog 1 (A. nidulans) |
| Calculated Molecular Weight | 335 aa, 38 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC001421 |
| Gene Symbol | NDE1 |
| Gene ID (NCBI) | 54820 |
| RRID | AB_2149877 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NXR1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NDE1 (NUDE /NUDE1) is a LIS1-interacting protein. A signaling pathway containing NDE1, LIS1, and cytoplasmic dynein is conserved for eukaryotic nuclear migration and has profound impact on neuronal migration during development of the central nervous system. LIS1 and dynein have been shown to be involved in mitosis and NDE1 participates in a subset of dynein function in M phase. NDE1 is specifically phosphorylated in M phase in its serine/threonine phosphorylation motifs, probably by Cdc2 and also Erk1 and Erk2. A fraction of NDEl binds to centrosomes strongly in interphase and is localized to mitotic spindles in early M phase. Phosphorylation of NDE1 also regulates the cell-cycle-dependent distribution, possibly by increasing its dissociation rate at the microtubule-organizing center. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human NDE1.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NDE1 antibody 10233-1-AP | Download protocol |
| IHC protocol for NDE1 antibody 10233-1-AP | Download protocol |
| IP protocol for NDE1 antibody 10233-1-AP | Download protocol |
| WB protocol for NDE1 antibody 10233-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NDE1 Polyclonal antibody (Cat# 10233-1-AP) has accumulated 43 citations in journals including Science, Nature Cell Biology, Nature Communications, Neuron, EMBO Journal, Cell Reports, Journal of Cell Biology, PLoS Biology, and eLife. Associated research fields encompass cell biology (spindle assembly, centrosome biology, ciliogenesis), neuroscience (neurogenesis, neuronal migration, cortical development), cell cycle regulation, neurodevelopmental and psychiatric disorders, immunology, and cancer biology. Primary applications include WB, IF, IHC, and IP.
| Species | Application | Title |
|---|---|---|
Science A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes. | ||
Nat Cell Biol Nde1-mediated inhibition of ciliogenesis affects cell cycle re-entry.
| ||
Protein Cell Neddylation inhibitor MLN4924 suppresses cilia formation by modulating AKT1. | ||
Nat Commun CBP-HSF2 structural and functional interplay in Rubinstein-Taybi neurodevelopmental disorder |
Reviews
What customers say
Both reviewers (ratings: 5/5 and 4/5) used this antibody for immunofluorescence and Western blot with positive results. One tested it on HeLa K cells at 1:500 dilution for IF, while the other used it on primary human fibroblasts at 1:1500 for WB and 1:100 for IF. Both used the same lot (00047462). Overall, users report reliable performance across cell types and applications, with clear staining observed in both fixed-cell IF and lysate-based WB experiments.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Philip (Verified Customer) (09-04-2019) | Cells fixed in PTEMF for 10min at RT. Blocked in PBS+3% BSA for 30min at RT. Primary at 1/500 for 1hr at RT.
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Aurelie (Verified Customer) (09-01-2019) | lysates (15µg) from human primary Fibroblasts were loaded in 8% SDS-PAGE and immunoblotted using anti-Nde1 ( 1/1500) human primary Fibroblasts were fixed in PFA 4% and stained using anti-Nde1 ( 1/100) over-night at 4°C followed by 1h at RT with a secondary anti-rabbit Alexa (594nM) .
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