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CoraLite®594-conjugated NDE1 Polyclonal antibody

NDE1 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-10233

Synonyms

FLJ20101, HOM TES 87, NDE1, NUDE, NUDE1



Tested Applications

Positive IF detected inhuman breast cancer tissue
For other applications, we recommend the unconjugated version of this antibody, 10233-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-10233 targets NDE1 in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDE1 fusion protein Ag0336
Full Name nudE nuclear distribution gene E homolog 1 (A. nidulans)
Calculated molecular weight 335 aa, 38 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC001421
Gene symbol NDE1
Gene ID (NCBI) 54820
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm / 614 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

NDE1 (NUDE /NUDE1) is a LIS1-interacting protein. A signaling pathway containing NDE1, LIS1, and cytoplasmic dynein is conserved for eukaryotic nuclear migration and has profound impact on neuronal migration during development of the central nervous system. LIS1 and dynein have been shown to be involved in mitosis and NDE1 participates in a subset of dynein function in M phase. NDE1 is specifically phosphorylated in M phase in its serine/threonine phosphorylation motifs, probably by Cdc2 and also Erk1 and Erk2. A fraction of NDEl binds to centrosomes strongly in interphase and is localized to mitotic spindles in early M phase. Phosphorylation of NDE1 also regulates the cell-cycle-dependent distribution, possibly by increasing its dissociation rate at the microtubule-organizing center. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human NDE1.

Protocols

Product Specific Protocols
IF protocol for CL594 NDE1 antibody CL594-10233Download protocol
Standard Protocols
Click here to view our Standard Protocols