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  • KD/KO Validated

NDE1 Polyclonal antibody

NDE1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP

Conjugate

Unconjugated

Cat no : 10233-1-AP

Synonyms

HOM TES 87, NDE1, NUDE, NUDE1



Tested Applications

Positive WB detected inA2780 cells, HEK-293 cells, mouse liver tissue, rat liver tissue, HeLa cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue, MCF-7 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

10233-1-AP targets NDE1 in WB, IP, IHC, IF, CoIP applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mosue, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDE1 fusion protein Ag0336
Full Name nudE nuclear distribution gene E homolog 1 (A. nidulans)
Calculated molecular weight 335 aa, 38 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC001421
Gene symbol NDE1
Gene ID (NCBI) 54820
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NDE1 (NUDE /NUDE1) is a LIS1-interacting protein. A signaling pathway containing NDE1, LIS1, and cytoplasmic dynein is conserved for eukaryotic nuclear migration and has profound impact on neuronal migration during development of the central nervous system. LIS1 and dynein have been shown to be involved in mitosis and NDE1 participates in a subset of dynein function in M phase. NDE1 is specifically phosphorylated in M phase in its serine/threonine phosphorylation motifs, probably by Cdc2 and also Erk1 and Erk2. A fraction of NDEl binds to centrosomes strongly in interphase and is localized to mitotic spindles in early M phase. Phosphorylation of NDE1 also regulates the cell-cycle-dependent distribution, possibly by increasing its dissociation rate at the microtubule-organizing center. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human NDE1.

Protocols

Product Specific Protocols
WB protocol for NDE1 antibody 10233-1-APDownload protocol
IHC protocol for NDE1 antibody 10233-1-APDownload protocol
IF protocol for NDE1 antibody 10233-1-APDownload protocol
IP protocol for NDE1 antibody 10233-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Protein Cell

Neddylation inhibitor MLN4924 suppresses cilia formation by modulating AKT1.

Authors - Hongmei Mao
humanWB

Ann Neurol

Extraciliary roles of the ciliopathy protein JBTS17 in mitosis and neurogenesis.

Authors - Hyowon Hong
mouseIF

PLoS Biol

NRF2 negatively regulates primary ciliogenesis and hedgehog signaling.

Authors - Pengfei Liu
mosueWB

Cereb Cortex Commun

Behavioral Deficits in Mice with Postnatal Disruption of Ndel1 in Forebrain Excitatory Neurons: Implications for Epilepsy and Neuropsychiatric Disorders.

Authors - Cezar Gavrilovici
humanWB, IHC

Cell Rep

Cilium induction triggers differentiation of glioma stem cells.

Authors - Gladiola Goranci-Buzhala
mouseWB

Life Sci Alliance

Loss of polycystins suppresses deciliation via the activation of the centrosomal integrity pathway.

Authors - Vasileios Gerakopoulos

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Philip (Verified Customer) (09-04-2019)

Cells fixed in PTEMF for 10min at RT. Blocked in PBS+3% BSA for 30min at RT. Primary at 1/500 for 1hr at RT.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HeLa K
FH

Aurelie (Verified Customer) (09-01-2019)

lysates (15µg) from human primary Fibroblasts were loaded in 8% SDS-PAGE and immunoblotted using anti-Nde1 ( 1/1500) human primary Fibroblasts were fixed in PFA 4% and stained using anti-Nde1 ( 1/100) over-night at 4°C followed by 1h at RT with a secondary anti-rabbit Alexa (594nM) .

  • Applications: Western Blot,Immunofluorescence,
  • Primary Antibody Dilution: 1/1500 (WB) et 1/100 (IF)
  • Cell Tissue Type: primary human fibroblasts
NDE1 Antibody Western Blot,Immunofluorescence, validation (1/1500 (WB) et 1/100 (IF) dilution) in primary human fibroblasts (Cat no:10233-1-AP)