NDRG1 Polyclonal antibody

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  WB analysis using 26902-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26902-1-AP (NDRG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 26902-1-AP

  WB result of NDRG1 antibody (26902-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDRG1 transfected HEK-293 cells.

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  WB analysis of HeLa using 26902-1-AP

  WB result of NDRG1 antibody (26902-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDRG1 transfected HeLa cells.

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  IHC staining of human colon cancer using 26902-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26902-1-AP (NDRG1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 26902-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26902-1-AP (NDRG1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 26902-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NDRG1 antibody (26902-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of HeLa using 26902-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human NDRG1 (26902-1-AP)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

• Featured Product
• KD/KO Validated

NDRG1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 26902-1-AP

Publications(24)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

CMT4D, Differentiation-related gene 1 protein, DRG1, DRG-1, Protein NDRG1

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Tested Applications

• Positive WB detected in: HEK-293T cells, HEK-293 cells, HeLa cells, Jurkat cells, mouse kidney tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 21 publications below
• IHC: See 7 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

26902-1-AP targets NDRG1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25424

  Product name: Recombinant human NDRG1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 45-109 aa of BC006260

  Sequence: SGSSVTSLDGTRSRSHTSEGTRSRSHTSEGTRSRSHTSEGAHLDITPNSGAAGNSAGPKSMEVSC

• Full Name: N-myc downstream regulated 1
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC006260
• Gene Symbol: NDRG1
• Gene ID (NCBI): 10397
• RRID: AB_2880676
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q92597
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NDRG1 is a member of the N-myc down-regulated gene family which belongs to the alpha/beta hydrolase superfamily. NDRG1 is a highly conserved cytoplasmic protein involved in cell growth arrest and proliferation, cell differentiation, DNA damage response, heavy metal response, the hypoxia response, tumorigenesis, and metastasis. NDRG1 is a potent metastasis suppressor in multiple tumor-types. NDRG1 appears to serve as a putative tumor suppressor and is down-regulated in colorectal, gastric, cervical, ovarian and prostate cancers.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	IHC,IF	Nat Aging; Single-cell and spatial RNA sequencing identify divergent microenvironments and progression signatures in early- versus late-onset prostate cancer; Authors - Yifei Cheng
human	WB	Mol Cell; mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.; Authors - Elodie Villa
human	WB	Nucleic Acids Res; TARG1 protects against toxic DNA ADP-ribosylation.; Authors - Callum Tromans-Coia ; KD Validated
human	WB,IHC	Cell Oncol (Dordr); LINC01419 promotes cell proliferation and metastasis in hepatocellular carcinoma by enhancing NDRG1 promoter activity.; Authors - Hao Dang
human	WB	Gynecol Oncol; Deferasirox shows inhibition activity against cervical cancer in vitro and in vivo.; Authors - Nan Zhou
human		Mol Biol Rep; Excessive iodine induces thyroid follicular epithelial cells apoptosis by activating HIF-1α-mediated hypoxia pathway in Hashimoto thyroiditis; Authors - Lili Zhang