Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IF/ICC detected in | SH-SY5Y cells, U-251 cells |
| Positive FC (Intra) detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19483-1-AP targets Nestin in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | nestin |
| Calculated Molecular Weight | 177 kDa |
| Observed Molecular Weight | 170-180 kDa |
| GenBank Accession Number | NM_006617 |
| Gene Symbol | Nestin |
| Gene ID (NCBI) | 10763 |
| RRID | AB_10644440 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P48681 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors. Nestin can be detected as 220-240 kDa and 240-280 kDa. Studies point toward nestin heavy posttranslational modifications as possibly serving particular functions in podocytes and in a few other particular cell types(PMID: 17210924).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Nestin antibody 19483-1-AP | Download protocol |
| IF protocol for Nestin antibody 19483-1-AP | Download protocol |
| WB protocol for Nestin antibody 19483-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Nestin Polyclonal antibody (Cat# 19483-1-AP) has 166 citations across 100+ journals, including Nature Communications, ACS Nano, Biomaterials, Stem Cell Research & Therapy, and Advanced Science. Research fields span neural stem cell biology, glioma/glioblastoma, spinal cord injury repair, neurogenesis, stroke, Alzheimer's disease, Parkinson's disease, iPSC characterization, dental pulp regeneration, and depression. Cited applications are primarily IF, WB, and IHC, with reactivity in human, mouse, rat, monkey, and zebrafish.
| Species | Application | Title |
|---|---|---|
Adv Mater Spinal Cord-Like Scaffold with Rapid Tissue Integration Enhanced Spinal Cord Nerve Repair. | ||
Bone Res CUL4B orchestrates mesenchymal stem cell commitment by epigenetically repressing KLF4 and C/EBPδ | ||
Autophagy FGF21 rejuvenates aged human adipose-derived mesenchymal stem cells via enhancement of TFE3-mediated autophagy flux. | ||
Nat Commun Hepatocyte growth factor derived from senescent cells attenuates cell competition-induced apical elimination of oncogenic cells | ||
ACS Nano Supramolecular Hydrogel Microspheres of Platelet-Derived Growth Factor Mimetic Peptide Promote Recovery from Spinal Cord Injury | ||
ACS Nano Nitric Oxide-Releasing Mesoporous Hollow Cerium Oxide Nanozyme-Based Hydrogel Synergizes with Neural Stem Cell for Spinal Cord Injury Repair |
Reviews
What customers say
Five of six reviewers rated this Nestin antibody 5 stars, praising its high specificity and reliable performance in Western blot and immunofluorescence across cell lines (MSCs, U2OS, iPSC-derived neural progenitors) and tissue. Recommended dilutions ranged from 1:100 to 1:1000 depending on application. One reviewer gave 2 stars, reporting inconclusive results in human brain cortical sections with a DAPI halo and no clear nestin signal. Overall, the antibody is well-regarded for WB and IF in cell-based assays, with mixed results in fixed tissue sections.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Cristine (Verified Customer) (11-09-2025) | The Nestin antibody worked very well and with high specificity at the dilution of 1:100 after overnight incubation at 4oC in immunocytochemistry assays of neural progenitor cells derived from human induced pluripotent stem cells.
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Udesh (Verified Customer) (08-12-2025) | Worked well for both IF and western
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Reyes (Verified Customer) (03-18-2024) | I tested nestin (in red) in human brain cortical sections obtained from pediatric patients, and the result was inconclusive, as it looks like ther is a halo around my DAPI, and there is no clear nestin marking in any cell type in the tissue.
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Udesh (Verified Customer) (02-24-2023) | Nestin in the upper lane Worked well at 1:1000 in 5% BSA, O/N, 4 degrees
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Balawant (Verified Customer) (08-23-2022) | this antibody is excellent for westernblot
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Silvia (Verified Customer) (09-30-2021) | Immunofluorescence (1:200) works well on NPC
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