Nestin Polyclonal antibody

Nestin Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 19483-1-AP

Synonyms

NES, Nestin



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman gliomas tissue, human kidney tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU-251 cells, rat brain tissue, SH-SY5Y cells, mouse brain tissue
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

19483-1-AP targets Nestin in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name nestin
Calculated molecular weight 177 kDa
Observed molecular weight 170-180 kDa
GenBank accession numberNM_006617
Gene symbol NES
Gene ID (NCBI) 10763
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.

Protocols

Product Specific Protocols
WB protocol for Nestin antibody 19483-1-APDownload protocol
IHC protocol for Nestin antibody 19483-1-APDownload protocol
IF protocol for Nestin antibody 19483-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IF

Brain Res

Brain-derived neurotrophic factor modified human umbilical cord mesenchymal stem cells-derived cholinergic-like neurons improves spatial learning and memory ability in Alzheimer's disease rats.

Authors - Weiwei Hu
humanIHC

Theranostics

Oncogenic MSH6-CXCR4-TGFB1 Feedback Loop: A Novel Therapeutic Target of Photothermal Therapy in Glioblastoma Multiforme.

Authors - Yaodong Chen
humanWB

J Nanobiotechnology

Mild thermotherapy and hyperbaric oxygen enhance sensitivity of TMZ/PSi nanoparticles via decreasing the stemness in glioma.

Authors - Xiaofan Zeng
ratIHC,IF

Biochem Biophys Res Commun

Nell-1 promotes the neural-like differentiation of dental pulp cells.

Authors - Qi Han
ratIHC

CNS Neurosci Ther

Transplantation of Nurr1-overexpressing neural stem cells and microglia for treating parkinsonian rats.

Authors - Yuan Qian
humanWB

Int J Oncol

Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells.

Authors - Wei Jia