Nestin Polyclonal antibody

Nestin Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 19483-1-AP


NES, Nestin

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman kidney tissue, human gliomas tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inrat brain tissue, SH-SY5Y cells, mouse brain tissue, U-251 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19483-1-AP targets Nestin in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name nestin
Calculated Molecular Weight 177 kDa
Observed Molecular Weight 170-180 kDa
GenBank Accession NumberNM_006617
Gene Symbol NES
Gene ID (NCBI) 10763
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors. Nestin can be detected as 220-240 kDa and 240-280 kDa. Studies point toward nestin heavy posttranslational modifications as possibly serving particular functions in podocytes and in a few other particular cell types(PMID: 17210924).


Product Specific Protocols
WB protocol for Nestin antibody 19483-1-APDownload protocol
IHC protocol for Nestin antibody 19483-1-APDownload protocol
IF protocol for Nestin antibody 19483-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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Supramolecular Hydrogel Microspheres of Platelet-Derived Growth Factor Mimetic Peptide Promote Recovery from Spinal Cord Injury

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Authors - Ze-Xu Wei

J Mater Chem B

Integration of genetically engineered virus nanofibers and fibrin to form injectable fibrous neuron-rich hydrogels and enable neural differentiation

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PLoS Pathog

Human cytomegalovirus pUL97 upregulates SOCS3 expression via transcription factor RFX7 in neural progenitor cells

Authors - Xian-Zhang Wang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Reyes (Verified Customer) (03-18-2024)

I tested nestin (in red) in human brain cortical sections obtained from pediatric patients, and the result was inconclusive, as it looks like ther is a halo around my DAPI, and there is no clear nestin marking in any cell type in the tissue.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Human Brain Cortex
Nestin Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:19483-1-AP)

Udesh (Verified Customer) (02-24-2023)

Nestin in the upper lane Worked well at 1:1000 in 5% BSA, O/N, 4 degrees

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: U2OS Osteosarcoma cells
Nestin Antibody Western Blot validation (1:1000 dilution) in U2OS Osteosarcoma cells (Cat no:19483-1-AP)

Balawant (Verified Customer) (08-23-2022)

this antibody is excellent for westernblot

  • Applications: Western Blot
  • Cell Tissue Type: colon tissue and colon cell line

Silvia (Verified Customer) (09-30-2021)

Immunofluorescence (1:200) works well on NPC

  • Applications: Immunofluorescence