NEUROG1 Monoclonal antibody

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  WB analysis of HEK-293 using 66642-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66642-1-Ig (NEUROG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 66642-1-Ig

  Y79 cells were subjected to SDS PAGE followed by western blot with 66642-1-Ig (NEUROG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 66642-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66642-1-Ig (NEUROG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66642-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66642-1-Ig (NEUROG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 66642-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66642-1-Ig (NEUROG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of rat brain using 66642-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NEUROG1 antibody (66642-1-Ig, Clone: 1E1H6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NEUROG1 Monoclonal Antibody for WB, IF-P, ELISA

Cat No. 66642-1-Ig

Clone No.1E1H6

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Synonyms

1E1H6, AKA, bHLHa6, Class A basic helix-loop-helix protein 6, Math4C

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Tested Applications

• Positive WB detected in: HEK-293 cells, Y79 cells, Neuro-2a cells, HeLa cells, mouse brain tissue
• Positive IF-P detected in: rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66642-1-Ig targets NEUROG1 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag26438

  Product name: Recombinant human NEUROG1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-237 aa of BC008687

  Sequence: MPARLETCISDLDCASSSGSDLSGFLTDEEDCARLQQAASASGPPAPARRGAPNISRASEVPGAQDDEQERRRRRGRTRVRSEALLHSLRRSRRVKANDRERNRMHNLNAALDALRSVLPSFPDDTKLTKIETLRFAYNYIWALAETLRLADQGLPGGGARERLLPPQCVPCLPGPPSPASDAESWGSGAAAASPLSDPSSPAASEDFTYRPGDPVFSFPSLPKDLLHTTPCFIPYH

• Full Name: neurogenin 1
• Calculated Molecular Weight: 237 aa, 26 kDa
• Observed Molecular Weight: 20-25 kDa
• GenBank Accession Number: BC008687
• Gene Symbol: NEUROG1
• Gene ID (NCBI): 4762
• RRID: AB_2882001
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q92886
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NEUROG1, also named BHLHA6, NEUROD3, NGN, and NGN1, is a 237 amino acid protein, which is expressed in embryonic nervous system. NEUROG1 acts as a transcriptional regulator that is involved in the initiation of neuronal differentiation. It activates transcription by binding to the E box (5'-CANNTG-3'). NEUROG1 can associate with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis.