Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, mouse spleen tissue, K-562 cells, Raji cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human cervical cancer tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 28 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
| IP | See 2 publications below |
| CoIP | See 4 publications below |
| RIP | See 8 publications below |
Product Information
19887-1-AP targets NF90/ILF3 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13707 Product name: Recombinant human NF90,ILF3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 8-343 aa of BC064836 Sequence: VNDDRHVMAKHSSVYPTQEELEAVQNMVSHTERALKAVSDWIDEQEKGSSEQAESDNMDVPPEDDSKEGAGEQKTEHMTRTLRGVMRVGLVAKGLLLKGDLDLELVLLCKEKPTTALLDKVADNLAIQLAAVTEDKYEILQSVDDAAIVIKNTKEPPLSLTIHLTSPVVREEMEKVLAGETLSVNDPPDVLDRQKCLAALASLRHAKWFQARANGLKSCVIVIRVLRDLCTRVPTWGPLRGWPLELLCEKSIGTANRPMGAGEALRRVLECLASGIVMPDGSGIYDPCEKEATDAIGHLDRQQREDITQSAQHALRLAAFGQLHKVLGMDPLPSKM Predict reactive species |
| Full Name | interleukin enhancer binding factor 3, 90kDa |
| Calculated Molecular Weight | 894 aa, 95 kDa |
| Observed Molecular Weight | 90 kDa, 110 kDa |
| GenBank Accession Number | BC064836 |
| Gene Symbol | ILF3 |
| Gene ID (NCBI) | 3609 |
| RRID | AB_10666431 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q12906 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Nuclear factor of activated T cells (NFAT)is a transcription factor required for T-cell expression of interleukin-2 (IL2). NFAT binds to a sequence known as the antigen receptor response element-2 (ARRE2) in the IL2 enhancer. NFAT contains two subunits, one is ILF3. ILF3 can facilitate double-standed RNA-regulated gene expression at the level of post-transcription. It binds to and inhibits coding sequences of acid beta-glucosidase and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. ILF3 exists main two isoforms NFAR-1 (90 kDa) and -2 (110 kDa). NFAR-1 and -2 each contain two DRBDs in their C-terminus regions and exhibit 98% homology to one another at the amino acid level. NFAR-2 contains an extra 192 amino acids at the C-terminal region that are encoded by an extra three exons present in its corresponding transcript.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NF90/ILF3 antibody 19887-1-AP | Download protocol |
| IHC protocol for NF90/ILF3 antibody 19887-1-AP | Download protocol |
| IP protocol for NF90/ILF3 antibody 19887-1-AP | Download protocol |
| WB protocol for NF90/ILF3 antibody 19887-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.
| ||
EMBO J Suppression of miR-199a maturation by HuR is crucial for hypoxia-induced glycolytic switch in hepatocellular carcinoma. | ||
Dev Cell Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia. | ||
Cancer Res CircFOXK2 Promotes Growth and Metastasis of Pancreatic Ductal Adenocarcinoma by Complexing with RNA Binding Proteins and Sponging MiR-942. | ||
Nucleic Acids Res Oxygen-dependent expression of cytochrome c oxidase subunit 4-2 gene expression is mediated by transcription factors RBPJ, CXXC5 and CHCHD2. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Praveen (Verified Customer) (03-13-2019) | Very Good
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FH SHUBHAM (Verified Customer) (02-25-2019) | eXCELLENT
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