Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, mouse spleen tissue, K-562 cells, Raji cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human cervical cancer tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19887-1-AP targets NF90/ILF3 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13707 Product name: Recombinant human NF90,ILF3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 8-343 aa of BC064836 Sequence: VNDDRHVMAKHSSVYPTQEELEAVQNMVSHTERALKAVSDWIDEQEKGSSEQAESDNMDVPPEDDSKEGAGEQKTEHMTRTLRGVMRVGLVAKGLLLKGDLDLELVLLCKEKPTTALLDKVADNLAIQLAAVTEDKYEILQSVDDAAIVIKNTKEPPLSLTIHLTSPVVREEMEKVLAGETLSVNDPPDVLDRQKCLAALASLRHAKWFQARANGLKSCVIVIRVLRDLCTRVPTWGPLRGWPLELLCEKSIGTANRPMGAGEALRRVLECLASGIVMPDGSGIYDPCEKEATDAIGHLDRQQREDITQSAQHALRLAAFGQLHKVLGMDPLPSKM Predict reactive species |
| Full Name | interleukin enhancer binding factor 3, 90kDa |
| Calculated Molecular Weight | 894 aa, 95 kDa |
| Observed Molecular Weight | 90 kDa, 110 kDa |
| GenBank Accession Number | BC064836 |
| Gene Symbol | ILF3 |
| Gene ID (NCBI) | 3609 |
| RRID | AB_10666431 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q12906 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Nuclear factor of activated T cells (NFAT)is a transcription factor required for T-cell expression of interleukin-2 (IL2). NFAT binds to a sequence known as the antigen receptor response element-2 (ARRE2) in the IL2 enhancer. NFAT contains two subunits, one is ILF3. ILF3 can facilitate double-standed RNA-regulated gene expression at the level of post-transcription. It binds to and inhibits coding sequences of acid beta-glucosidase and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. ILF3 exists main two isoforms NFAR-1 (90 kDa) and -2 (110 kDa). NFAR-1 and -2 each contain two DRBDs in their C-terminus regions and exhibit 98% homology to one another at the amino acid level. NFAR-2 contains an extra 192 amino acids at the C-terminal region that are encoded by an extra three exons present in its corresponding transcript.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NF90/ILF3 antibody 19887-1-AP | Download protocol |
| IHC protocol for NF90/ILF3 antibody 19887-1-AP | Download protocol |
| IP protocol for NF90/ILF3 antibody 19887-1-AP | Download protocol |
| WB protocol for NF90/ILF3 antibody 19887-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-ILF3 antibody (Cat# 19887-1-AP) has 47 citations spanning journals including Cell, Nature Cell Biology, Nature Communications, Molecular Cell, Cancer Research, Nucleic Acids Research, EMBO Journal, Cell Reports, and Developmental Cell. Research fields cover RNA biology, oncology (colorectal, pancreatic, liver, lung, breast cancers), cardiovascular disease, neurology, mitochondrial biology, immunology, epigenetics, and ferroptosis, with applications in WB, RIP, IP, CoIP, IF, and IHC.
| Species | Application | Title |
|---|---|---|
Cell In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.
| ||
Mol Cancer Circular RNA circPHLPP2 promotes tumor growth and anti-PD-1 resistance through binding ILF3 to regulate IL36γ transcription in colorectal cancer. | ||
Nat Cell Biol Genome-wide CRISPR screens identify ILF3 as a mediator of mTORC1-dependent amino acid sensing
| ||
Cancer Res CircFOXK2 Promotes Growth and Metastasis of Pancreatic Ductal Adenocarcinoma by Complexing with RNA Binding Proteins and Sponging MiR-942. | ||
Nat Commun Macrophage ILF3 promotes abdominal aortic aneurysm by inducing inflammatory imbalance in male mice |
Reviews
What customers say
Both reviewers used this antibody for Western Blot and reported positive results. One tested it on HEK293 cells and rated it 4 out of 5, describing the performance as "very good." The other tested it on Ac16 cells and gave it a perfect 5 out of 5, calling the result "excellent." Overall, users found the antibody reliable and effective for WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Praveen (Verified Customer) (03-13-2019) | Very Good
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SHUBHAM (Verified Customer) (02-25-2019) | eXCELLENT
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