Recombinant human NGF protein

Source

e coli.-derived, PET28a

Tag

6*His

Format

Liquid

Publications

2

Cat no : Ag25370

Synonyms

Beta NGF, NGF, Beta nerve growth factor, Beta-nerve growth factor, Beta-NGF

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Validation Data Gallery View All

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  NGF fusion protein Ag25370 SDS-PAGE

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Product Information

• Peptide Sequence: (1-299 aa encoded by BC032517)
• Activity: Not tested.
• Endotoxin Level: Please contact the lab for more information.
• Formulation: The protein was expressed as 6xHis-tagged fusion protein by E.Coli and purified by Ni-sepharose. The purified protein was resolved in PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4 ) added with 15% glycerol. The elution buffer contain 300mM imidazole.

Shipping and Storage

Reconstitution	Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage. After reconstitution with sterile water, if glycerol has no effect on subsequent experiments, it is recommended to add an equal volume of glycerol for long-term storage (see Stability and Storage for more details). If a different concentration is needed for your purposes please adjust the reconstitution volume as required (please note: the ion concentration of the final solution will vary according to the volume used). Note: Centrifuge vial before opening. When reconstituting, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution.
Shipping	The product is shipped with ice packs. Upon receipt, store it immediately at -20°C to -80°C.
Stability and Storage	Aliquot and store at -20°C to -80°C for up to 6 months. Avoid freeze thaw cycles.
Storage of Reconstituted Protein	Short-term storage: Store at 2-8°C for (1-2 weeks). Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, reconstitution with sterile water and addition of an equal volume of glycerol. Avoid repeat freeze-thaw cycles.

Publications

Species	Title
	J Exp Clin Cancer Res; Hedgehog-Gli1-derived exosomal circ-0011536 mediates peripheral neural remodeling in pancreatic cancer by modulating the miR-451a/VGF axis; Authors - Weiqi Dai
	J Orthop Surg Res; Pain mediator NGF improves chondrocyte extracellular matrix synthesis via PI3K/AKT pathway; Authors - Mengling Wang