NINJ2 Monoclonal antibody

NINJ2 Monoclonal Antibody for IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

5C5B2

Cat no : 66202-1-Ig

Synonyms

Nerve injury induced protein 2, NINJ2, ninjurin 2

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Validation Data Gallery

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  IHC staining of human tonsillitis using 66202-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66202-1-Ig (NINJ2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 66202-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66202-1-Ig (NINJ2 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of human tonsillitis using 66202-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using NINJ2 antibody (66202-1-Ig, Clone: 5C5B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human tonsillitis tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66202-1-Ig targets NINJ2 in IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: NINJ2 fusion protein Ag5343
• Full Name: ninjurin 2
• Calculated Molecular Weight: 142 aa, 16 kDa
• GenBank Accession Number: BC057766
• Gene Symbol: NINJ2
• Gene ID (NCBI): 4815
• RRID: AB_2881593
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NINJ2 (Ninjurin2) is a member of the ninjurin family of adhesion molecules which mediate cell-to-cell and cell-to-extracellular matrix interactions during development, differentiation, and regeneration of the peripheral nervous system. The gene encodes NINJ2 is located on chromosome 12p13. NINJ2 mRNA is widely expressed in adult human tissues, with highest level in bone marrow, followed by peripheral leukocytes, lung, and lymph nodes. In the peripheral nervous system, NINJ2 is expressed constitutively in mature sensory and enteric neurons. The expression of NINJ2 is upregulated after nerve injury in Schwann cells, suggesting that it may promote nerve regeneration. It may also play an important role in the pathogenesis of inflammatory disorder. (PMID: 10627596)