NIT1 Polyclonal antibody

• Featured Product
• KD/KO Validated

NIT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14380-1-AP

Synonyms

Nitrilase homolog 1, nitrilase 1, EC:3.5.1.128, dGSH amidase, Deaminated glutathione amidase

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Validation Data Gallery

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  WB analysis using 14380-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14380-1-AP (NIT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14380-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14380-1-AP (NIT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 14380-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 14380-1-AP (NIT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 14380-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14380-1-AP (NIT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 14380-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14380-1-AP (NIT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 14380-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14380-1-AP (NIT1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 14380-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14380-1-AP (NIT1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 14380-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14380-1-AP (NIT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, HEK-293T cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

14380-1-AP targets NIT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NIT1 fusion protein Ag5764
• Full Name: nitrilase 1
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 32-36 kDa
• GenBank Accession Number: BC046149
• Gene Symbol: NIT1
• Gene ID (NCBI): 4817
• RRID: AB_2151314
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Cell Death Dis; NIT1 suppresses tumour proliferation by activating the TGFβ1-Smad2/3 signalling pathway in colorectal cancer.; Authors - Chun Lin ; KD Validated