NKD2 Polyclonal antibody, PBS Only

• 

  WB analysis of mouse kidney using 16699-1-AP (same clone as 16699-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16699-1-AP (NKD2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16699-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 16699-1-AP (same clone as 16699-1-PBS)

  WB result of NKD2 antibody (16699-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-NKD2 transfected HEK-293 cells. This data was developed using the same antibody clone with 16699-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 16699-1-AP (same clone as 16699-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16699-1-AP (NKD2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16699-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse colon using 16699-1-AP (same clone as 16699-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 16699-1-AP (NKD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16699-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

NKD2 Polyclonal Antibody for WB, Indirect ELISA

Cat No. 16699-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Naked-2, Naked2, Naked 2, hNkd2

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Product Information

16699-1-PBS targets NKD2 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10094

  Product name: Recombinant human Naked2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-311 aa of BC012176

  Sequence: MGKLQSKHAAAARKRRESPEGDSFVASAYASGRKGAEEAERRARDKQELPNGDPKEGPFREDQCPLQVALPAEKAEGREHPGQLLSADDGERAANREGPRGPGGQRLNIDALQCDVSVEEDDRQEWTFTLYDFDNCGKVTREDMSSLMHTIYEVVDASVNHSSGSSKTLRVKLTVSPEPSSKRKEGPPAGQDREPTRCRMEGELAEEPRVADRRLSAHVRRPSTDPQPCSERGPYCVDENTERRNHYLDLAGIENYTSRFGPGSQLCEKRSSAPRTHSGDKARGVGLCRELWSQAGHPQWPGPFPSGLVAV

• Full Name: naked cuticle homolog 2 (Drosophila)
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC012176
• Gene Symbol: NKD2
• Gene ID (NCBI): 85409
• RRID: AB_2151468
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q969F2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Naked2 (protein naked cuticle homolog 2), encoded by NKD2 gene, was identified as a cell autonomous antagonist of the canonical Wnt signaling pathway. Naked2 acts as a cargo recognition and targeting protein to ensure proper delivery and fusion of TGF-alpha-containing vesicles to a distinct region at the basolateral surface of polarized epithelial cells (PMID: 17553928). EGFR-independent action of TGF-alpha protects Naked2 from proteasomal degradation, thus ensuring its delivery to the basolateral surface (PMID: 18757723). Myristoylated Naked2 antagonizes Wnt-beta-catenin activity possibly by degrading Dishevelled-1 at the plasma membrane (PMID: 20177058).