NLRC3 Polyclonal antibody

NLRC3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30455-1-AP

Synonyms

CARD15 like protein, Caterpiller protein 16.2, CLR16.2, FLJ00348, NLRC3, NOD3, Protein NLRC3

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Validation Data Gallery

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  WB analysis using 30455-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30455-1-AP (NLRC3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Daudi cells, Jurkat cells, Raji cells, Ramos cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30455-1-AP targets NLRC3 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NLRC3 fusion protein Ag32323
• Full Name: NLR family, CARD domain containing 3
• Calculated Molecular Weight: 115 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: NM_178844
• Gene Symbol: NLRC3
• Gene ID (NCBI): 197358
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NLRC3 is a member of the pattern recognition receptors nucleotide-binding oligomerization domain (NOD)-like receptors (NLRs) family, and plays a pivotal regulatory role in modulating the activation of immune cells. In macrophages, NLRC3 inhibits the activation of the NF-κB signaling pathway, the STING/TBK1 signaling pathway, and the formation of the inflammasome. In the context of T cells immune response, NLRC3 prevents the activation of T cells by regulating the function of dendritic cells and directly influencing the function of T cells.