NLRP12 Polyclonal antibody

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  WB analysis using 30998-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30998-1-AP (NLRP12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of rat liver using 30998-1-AP

  IP result of anti-NLRP12 (IP:30998-1-AP, 4ug; Detection:30998-1-AP 1:500) with rat liver tissue lysate 2695 ug.

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  IP experiment of PC-3 using 30998-1-AP

  IP result of anti-NLRP12 (IP:30998-1-AP, 4ug; Detection:30998-1-AP 1:500) with PC-3 cells lysate 1575 ug.

NLRP12 Polyclonal Antibody for WB, IP, ELISA

Cat No. 30998-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

PYPAF7, PAN6, NALP12, NACHT, LRR and PYD domains-containing protein 12, Monarch-1

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue
• Positive IP detected in: rat liver tissue, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30998-1-AP targets NLRP12 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag34281

  Product name: Recombinant human NLRP12 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 30-140 aa of BC028069

  Sequence: LYLGTATELGEGKIPWGSMEKAGPLEMAQLLITHFGPEEAWRLALSTFERINRKDLWERGQREDLVRDTPPGGPSSLGNQSTCLLEVSLVTPRKDPQETYRDYVRRKFRLM

• Full Name: NLR family, pyrin domain containing 12
• Calculated Molecular Weight: 1061 aa, 120 kDa
• Observed Molecular Weight: 95-110 kDa
• GenBank Accession Number: BC028069
• Gene Symbol: NLRP12
• Gene ID (NCBI): 91662
• RRID: AB_3669811
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P59046
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NACHT, LRR and PYD domains-containing protein 12 (NLRP12) is also named as Monarch-1, NALP12, PYPAF7 and RNO. NLRP12 is as an essential cytosolic sensor for heme plus PAMPs-mediated PANoptosis, inflammation, and pathology (PMID: 37267949). NLRP12 drives inflammasome and PANoptosome activation, cell death, and inflammation in response to heme plus PAMPs or TNF. TLR2/4-mediated signaling through IRF1 induced Nlrp12 expression, which led to inflammasome formation to induce maturation of IL-1β and IL-18 (PMID: 37267949). NLRP12, a pyrin-containing NLR protein, is a negative regulator of innate immune activation and type I interferon (IFN-I) production (PMID: 36719379).