iNOS Polyclonal antibody

iNOS Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

32

Cat no : 22226-1-AP

Synonyms

HEP NOS, Hepatocyte NOS, Inducible NO synthase, Inducible NOS, INOS, NOS, NOS type II, NOS2, NOS2A

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Validation Data Gallery

• 

  WB analysis using 22226-1-AP

  Untreated and LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 22226-1-AP (NOS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  FC experiment of RAW 264.7 using 22226-1-AP

  1X10^6 LPS treated RAW 264.7 cells were intracellularly stained with 0.4 ug Anti-Human iNOS (22226-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: RAW 264.7 cells
• Positive FC detected in: LPS treated RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 24 publications below
• IHC: See 3 publications below
• IF: See 6 publications below

Product Information

The immunogen of 22226-1-AP is iNOS Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: iNOS fusion protein Ag17567
• Full Name: nitric oxide synthase 2, inducible
• Calculated molecular weight: 1153 aa, 131 kDa
• Observed molecular weight: 110-130 kDa, 65-70 kDa
• GenBank accession number: BC130283
• Gene symbol: NOS2
• Gene ID (NCBI): 4843
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NOS2, also named as iNOS and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission, antimicrobial and antitumoral activities. NOS2 is a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. iNOS has a very short half-life due to rapid degradation by calpain. iNOS monomer is a direct substrate of calpain I and can be cleaved by calpain I at the canonical CaM-binding site(503-532aa) of iNOS, and then a ~70kD band can be detected by western (PMID:11786228).

Publications

Species	Application	Title
rat	IF	J Neuroinflammation; Apelin alleviated neuroinflammation and promoted endogenous neural stem cell proliferation and differentiation after spinal cord injury in rats.; Authors - Qing Liu
mouse	WB	J Neuroinflammation; The circadian clock protein Rev-erbα provides neuroprotection and attenuates neuroinflammation against Parkinson's disease via the microglial NLRP3 inflammasome.; Authors - Liang Kou
mouse	IF	J Genet Genomics; Fecal microbiota transplantation from young donor mice improves ovarian function in aged mice.; Authors - Li Xu
mouse	WB	Cells; Isoalantolactone (IAL) Regulates Neuro-Inflammation and Neuronal Apoptosis to Curb Pathology of Parkinson's Disease; Authors - Dewei He
rat	IHC	Phytomedicine; Protective effects and potential mechanism of salvianolic acid B on sodium laurate-induced thromboangiitis obliterans in rats.; Authors - Ziru Zhang
rat	WB	Front Pharmacol; Targeting Myadm to Intervene Pulmonary Hypertension on Rats Before Pregnancy Alleviates the Effect on Their Offspring's Cardiac-Cerebral Systems.; Authors - Jingrong Wang