- Featured Product
- KD/KO Validated
NOTCH3 Polyclonal antibody
NOTCH3 Polyclonal Antibody for FC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse and More (1)
WB, IP, IHC, IF, FC, ELISA
Cat no : 55114-1-AP
Validation Data Gallery
|Positive WB detected in||A2780 cells, Caco-2 cells, U2OS cells, COLO 320 cells, HeLa cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human lung cancer tissue, human breast cancer tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||Jurkat cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 4 publications below|
|WB||See 33 publications below|
|IHC||See 11 publications below|
|IF||See 3 publications below|
55114-1-AP targets NOTCH3 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||Notch homolog 3 (Drosophila)|
|Calculated molecular weight||244 kDa|
|Observed molecular weight||250-260 kDa, 100 kDa|
|GenBank accession number||NM_000435|
|Gene ID (NCBI)||4854|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
NOTCH3 belongs to the NOTCH family. It functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. NOTCH3 affects the implementation of differentiation, proliferation and apoptotic programs. Defects in NOTCH3 are the cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). The antibody is specific to NOTCH3. Notch3 is a single-pass type I membrane protein exposed at the cell surface as a heterodimer of 2321 amino acids. It is synthesized as a precursor with a molecular weight of ~280 kDa (Notch3 full-length), which is cleaved (S1 cleavage) into a 210 kDa extracellular fragment and a 97 kDa intracellular fragment (PMID: 21702048).
|Product Specific Protocols|
|WB protocol for NOTCH3 antibody 55114-1-AP||Download protocol|
|IHC protocol for NOTCH3 antibody 55114-1-AP||Download protocol|
|IP protocol for NOTCH3 antibody 55114-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Sci Transl Med
JAGGED-NOTCH3 signaling in vascular remodeling in pulmonary arterial hypertension.
METTL3-mediated m6A modification of TIMP2 mRNA promotes podocyte injury in diabetic nephropathy.
Laminin-521 promotes quiescence in isolated stellate cells from rat liver.
Manic fringe promotes a claudin-low breast cancer phenotype through notch-mediated PIK3CG induction.
Notch3 promotes prostate cancer-induced bone lesion development via MMP-3.
Lunatic Fringe is a potent tumor suppressor in Kras-initiated pancreatic cancer.