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NOTCH3 Polyclonal antibody

NOTCH3 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 55114-1-AP

Synonyms

CADASIL, CASIL, Notch 3, Notch homolog 3 (Drosophila), NOTCH3



Tested Applications

Positive WB detected inA2780 cells, Caco-2 cells, U2OS cells, COLO 320 cells, HeLa cells, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

55114-1-AP targets NOTCH3 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name Notch homolog 3 (Drosophila)
Calculated molecular weight 244 kDa
Observed molecular weight 250-260 kDa, 100 kDa
GenBank accession numberNM_000435
Gene symbol NOTCH3
Gene ID (NCBI) 4854
RRIDAB_10858393
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NOTCH3 belongs to the NOTCH family. It functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. NOTCH3 affects the implementation of differentiation, proliferation and apoptotic programs. Defects in NOTCH3 are the cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). The antibody is specific to NOTCH3. Notch3 is a single-pass type I membrane protein exposed at the cell surface as a heterodimer of 2321 amino acids. It is synthesized as a precursor with a molecular weight of ~280 kDa (Notch3 full-length), which is cleaved (S1 cleavage) into a 210 kDa extracellular fragment and a 97 kDa intracellular fragment (PMID: 21702048).

Protocols

Product Specific Protocols
WB protocol for NOTCH3 antibody 55114-1-APDownload protocol
IHC protocol for NOTCH3 antibody 55114-1-APDownload protocol
IP protocol for NOTCH3 antibody 55114-1-APDownload protocol
FC protocol for NOTCH3 antibody 55114-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Metab

Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.

Authors - Xiuying Zhong
human,mouse,ratWB,IF

Sci Transl Med

JAGGED-NOTCH3 signaling in vascular remodeling in pulmonary arterial hypertension.

Authors - Yu Zhang
mouseWB

Mol Ther

METTL3-mediated m6A modification of TIMP2 mRNA promotes podocyte injury in diabetic nephropathy.

Authors - Ling Jiang
ratWB

Biomaterials

Laminin-521 promotes quiescence in isolated stellate cells from rat liver.

Authors - Friederike Rohn
mouseWB

Cancer Res

Manic fringe promotes a claudin-low breast cancer phenotype through notch-mediated PIK3CG induction.

Authors - Shubing Zhang
humanWB

Oncogene

Notch3 promotes prostate cancer-induced bone lesion development via MMP-3.

Authors - Sourik S Ganguly
  • KD Validated