Validation Data Gallery
|Positive WB detected in
|A2780 cells, Caco-2 cells, U2OS cells, COLO 320 cells, HeLa cells, K-562 cells
|Positive IP detected in
|Positive IHC detected in
|human lung cancer tissue, human breast cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in
|Western Blot (WB)
|WB : 1:500-1:2000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
55114-1-AP targets NOTCH3 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|Notch homolog 3 (Drosophila)
|Calculated molecular weight
|Observed molecular weight
|250-260 kDa, 100 kDa
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
NOTCH3 belongs to the NOTCH family. It functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. NOTCH3 affects the implementation of differentiation, proliferation and apoptotic programs. Defects in NOTCH3 are the cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). The antibody is specific to NOTCH3. Notch3 is a single-pass type I membrane protein exposed at the cell surface as a heterodimer of 2321 amino acids. It is synthesized as a precursor with a molecular weight of ~280 kDa (Notch3 full-length), which is cleaved (S1 cleavage) into a 210 kDa extracellular fragment and a 97 kDa intracellular fragment (PMID: 21702048).
|Product Specific Protocols
|WB protocol for NOTCH3 antibody 55114-1-AP
|IHC protocol for NOTCH3 antibody 55114-1-AP
|IP protocol for NOTCH3 antibody 55114-1-AP
|FC protocol for NOTCH3 antibody 55114-1-AP
|Click here to view our Standard Protocols
Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.
Sci Transl Med
JAGGED-NOTCH3 signaling in vascular remodeling in pulmonary arterial hypertension.
METTL3-mediated m6A modification of TIMP2 mRNA promotes podocyte injury in diabetic nephropathy.
Laminin-521 promotes quiescence in isolated stellate cells from rat liver.
Manic fringe promotes a claudin-low breast cancer phenotype through notch-mediated PIK3CG induction.
Notch3 promotes prostate cancer-induced bone lesion development via MMP-3.