NOV/CCN3 Recombinant antibody

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  WB analysis using 85731-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85731-1-RR (Nov antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85731-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85731-1-RR (Nov antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85731-1-RR

  Mouse brain tissue was subjected to SDS PAGE followed by western blot with 85731-1-RR (Nov antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

NOV/CCN3 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85731-1-RR

Clone No.250054B2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

CCN family member 3, Ccn3, Cellular communication network factor 3, Nephroblastoma-overexpressed gene protein homolog, Nov

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Tested Applications

• Positive WB detected in: A172 cells, HEK-293T cells, mouse brain tissue, HeLa cells, mouse thymus tissue, Caco-2 cells, rat thymus tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85731-1-RR targets NOV/CCN3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NOV/CCN3 fusion protein Eg2856
• Full Name: nephroblastoma overexpressed gene
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: NM_010930.4
• Gene Symbol: Nov
• Gene ID (NCBI): 18133
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q64299
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCN family member 3 (CCN3), also known as Nephroblastoma overexpressed (NOV) protein, is a member of the CCN protein family (PMID: 1309586). CCN3 is predominantly expressed in the cytoplasm, nucleus, and extracellular space. As a subset of extracellular matrix proteins, it can directly bind to the integrin receptor family on the cell surface and participate in signal transduction between the cell surface and the extracellular matrix structure (PMID: 37378812). CCN3 exhibits anti-proliferative activity in normal cells and most tumor cells (PMID: 17340618). CCN3 co-localizes with connexin 43 (C×43) in the plasma membrane, and these two proteins physically interact to inhibit cell growth (PMID: 15213231). CCN3 can induce myofibroblast differentiation through the Notch pathway (PMID: 12050162). CCN can promote adhesion of endothelial cells, vascular smooth muscle cells, and fibroblasts through heparan sulfate proteoglycans (HSPG) (PMID: 30158025).