NPHP5/IQCB1 Polyclonal antibody

NPHP5/IQCB1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG







Cat no : 15747-1-AP


IQ motif containing B1, IQCB1, KIAA0036, Nephrocystin 5, NPHP5, NPHP5,IQCB1, PIQ, SLSN5

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

15747-1-AP targets NPHP5/IQCB1 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NPHP5/IQCB1 fusion protein Ag8203
Full Name IQ motif containing B1
Calculated Molecular Weight 598 aa, 69 kDa
Observed Molecular Weight69 kDa
GenBank Accession NumberBC005806
Gene Symbol IQCB1
Gene ID (NCBI) 9657
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IQCB1, also known as NPHP5, is a nephrocystin protein that interacts with calmodulin and the retinitis pigmentosa GTPase regulator protein. It has a central coiled-coil region and two calmodulin-binding IQ domains. Localized to the primary cilia of renal epithelial cells and connecting cilia of photoreceptor cells, IQCB1 is thought to play a role in ciliary function. Mutations in this gene result in Senior-Loken syndrome type 5, a juvenile disorder characterized by defects in the waste filtering system of the kidney, as well as retinal degradation.


Product Specific Protocols
WB protocol for NPHP5/IQCB1 antibody 15747-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Hum Mol Genet

Pathogenic NPHP5 mutations impair protein interaction with Cep290, a prerequisite for ciliogenesis.

Authors - Barbelanne Marine M

Mol Biol Cell

Rpgrip1l controls ciliary gating by ensuring the proper amount of Cep290 at the vertebrate transition zone.

Authors - Antonia Wiegering