BNP Polyclonal antibody

BNP Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 13299-1-AP

Synonyms

BNP, BNP(1 32), Natriuretic peptides B, NPPB

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Validation Data Gallery

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  IHC staining of mouse brain using 13299-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13299-1-AP (BNP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13299-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13299-1-AP (BNP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 13299-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13299-1-AP (BNP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 13299-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 13299-1-AP (BNP Antibody) at dilution of 1:50.

• 

  IHC staining of human brain using 13299-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 13299-1-AP (BNP Antibody) at dilution of 1:50.

• 

  IHC staining of human heart using 13299-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 13299-1-AP (BNP Antibody) at dilution of 1:50.

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  IHC staining of human heart using 13299-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 13299-1-AP (BNP Antibody) at dilution of 1:50.

• 

  IF Staining of rat brain using 13299-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using BNP antibody (13299-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 13299-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BNP antibody (13299-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive IHC detected in: mouse brain tissue, human brain tissue, human heart tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: rat brain tissue, HepG2 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• IHC: See 2 publications below

Product Information

13299-1-AP targets BNP in IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BNP fusion protein Ag4039
• Full Name: natriuretic peptide precursor B
• Calculated molecular weight: 134 aa, 15 kDa
• GenBank accession number: BC025785
• Gene symbol: NPPB
• Gene ID (NCBI): 4879
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NPPB, also known as B-type natriuretic peptide (BNP), is a cardiac hormone that is secreted mainly in the ventricles in response to increased wall stress. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. BNP is a marker of systolic and diastolic dysfunction and a strong predictor of mortality in heart failure patients.

Publications

Species	Application	Title
rat		Front Cardiovasc Med; Effects of Excessive Activation of N-methyl-D-aspartic Acid Receptors in Neonatal Cardiac Mitochondrial Dysfunction Induced by Intrauterine Hypoxia.; Authors - Yang Liu
		Pharm Biol; Epigallocatechin gallate (EGCG) attenuates myocardial hypertrophy and fibrosis induced by transverse aortic constriction via inhibiting the Akt/mTOR pathway.; Authors - Yue Cui
		Int Heart J; Phenotype and Functional Analyses in a Transgenic Mouse Model of Left Ventricular Noncompaction Caused by a DTNA Mutation.; Authors - Qing Cao
rat		Cell Regen; Fabrication of SA/Gel/C scaffold with 3D bioprinting to generate micro-nano porosity structure for skin wound healing: a detailed animal in vivo study.; Authors - Changmei Niu
	IHC	Front Pharmacol; Administration of USP7 inhibitor P22077 inhibited cardiac hypertrophy and remodeling in Ang II-induced hypertensive mice; Authors - Yu-Hui Gu
rat		Mol Med Rep; G9a inhibition promotes the formation of pacemaker-like cells by reducing the enrichment of H3K9me2 in the HCN4 promoter region; Authors - Pei Xu