BNP Polyclonal antibody

BNP Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, rat, mouse





Cat no : 13299-1-AP


BNP, BNP(1 32), Natriuretic peptides B, NPPB

Tested Applications

Positive IHC detected inmouse brain tissue, human brain tissue, human heart tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inrat brain tissue, HepG2 cells

Recommended dilution

Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 2 publications below

Product Information

13299-1-AP targets BNP in IF, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BNP fusion protein Ag4039
Full Name natriuretic peptide precursor B
Calculated Molecular Weight 134 aa, 15 kDa
GenBank Accession NumberBC025785
Gene Symbol NPPB
Gene ID (NCBI) 4879
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NPPB, also known as B-type natriuretic peptide (BNP), is a cardiac hormone that is secreted mainly in the ventricles in response to increased wall stress. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. BNP is a marker of systolic and diastolic dysfunction and a strong predictor of mortality in heart failure patients.


Product Specific Protocols
IHC protocol for BNP antibody 13299-1-APDownload protocol
IF protocol for BNP antibody 13299-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Front Cardiovasc Med

Effects of Excessive Activation of N-methyl-D-aspartic Acid Receptors in Neonatal Cardiac Mitochondrial Dysfunction Induced by Intrauterine Hypoxia.

Authors - Yang Liu

Pharm Biol

Epigallocatechin gallate (EGCG) attenuates myocardial hypertrophy and fibrosis induced by transverse aortic constriction via inhibiting the Akt/mTOR pathway.

Authors - Yue Cui

Int Heart J

Phenotype and Functional Analyses in a Transgenic Mouse Model of Left Ventricular Noncompaction Caused by a DTNA Mutation.

Authors - Qing Cao

Cell Regen

Fabrication of SA/Gel/C scaffold with 3D bioprinting to generate micro-nano porosity structure for skin wound healing: a detailed animal in vivo study.

Authors - Changmei Niu

Front Pharmacol

Administration of USP7 inhibitor P22077 inhibited cardiac hypertrophy and remodeling in Ang II-induced hypertensive mice

Authors - Yu-Hui Gu

Mol Med Rep

G9a inhibition promotes the formation of pacemaker-like cells by reducing the enrichment of H3K9me2 in the HCN4 promoter region

Authors - Pei Xu