BNP Polyclonal antibody

BNP Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human and More (1)





Cat no : 13299-1-AP


BNP, BNP(1 32), Natriuretic peptides B, NPPB

Tested Applications

Positive IHC detected inmouse brain tissue, human brain tissue, human heart tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inrat brain tissue, HepG2 cells

Recommended dilution

Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 2 publications below

Product Information

13299-1-AP targets BNP in IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BNP fusion protein Ag4039
Full Name natriuretic peptide precursor B
Calculated molecular weight 134 aa, 15 kDa
GenBank accession numberBC025785
Gene symbol NPPB
Gene ID (NCBI) 4879
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NPPB, also known as B-type natriuretic peptide (BNP), is a cardiac hormone that is secreted mainly in the ventricles in response to increased wall stress. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. BNP is a marker of systolic and diastolic dysfunction and a strong predictor of mortality in heart failure patients.


Product Specific Protocols
IHC protocol for BNP antibody 13299-1-APDownload protocol
IF protocol for BNP antibody 13299-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Front Cardiovasc Med

Effects of Excessive Activation of N-methyl-D-aspartic Acid Receptors in Neonatal Cardiac Mitochondrial Dysfunction Induced by Intrauterine Hypoxia.

Authors - Yang Liu

Pharm Biol

Epigallocatechin gallate (EGCG) attenuates myocardial hypertrophy and fibrosis induced by transverse aortic constriction via inhibiting the Akt/mTOR pathway.

Authors - Yue Cui

Int Heart J

Phenotype and Functional Analyses in a Transgenic Mouse Model of Left Ventricular Noncompaction Caused by a DTNA Mutation.

Authors - Qing Cao

Cell Regen

Fabrication of SA/Gel/C scaffold with 3D bioprinting to generate micro-nano porosity structure for skin wound healing: a detailed animal in vivo study.

Authors - Changmei Niu

Front Pharmacol

Administration of USP7 inhibitor P22077 inhibited cardiac hypertrophy and remodeling in Ang II-induced hypertensive mice

Authors - Yu-Hui Gu

Mol Med Rep

G9a inhibition promotes the formation of pacemaker-like cells by reducing the enrichment of H3K9me2 in the HCN4 promoter region

Authors - Pei Xu