Filter: AllWBIPIHC

WB analysis of HeLa using 10157-1-AP (same clone as 10157-1-PBS)
WB result of TUSC4 antibody (10157-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TUSC4 transfected HeLa cells. This data was developed using the same antibody clone with 10157-1-PBS in a different storage buffer formulation.
WB analysis of mouse pancreas using 10157-1-AP (same clone as 10157-1-PBS)
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 10157-1-AP (TUSC4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10157-1-PBS in a different storage buffer formulation.
IP experiment of mouse pancreas using 10157-1-AP (same clone as 10157-1-PBS)
IP result of anti-NPRL2 (IP:10157-1-AP, 3ug; Detection:10157-1-AP 1:500) with mouse pancreas tissue lysate 4000ug. This data was developed using the same antibody clone with 10157-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 10157-1-AP (same clone as 10157-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 10157-1-AP (NPR2L,TUSC4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 10157-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 10157-1-AP (same clone as 10157-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 10157-1-AP (NPR2L,TUSC4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 10157-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas using 10157-1-AP (same clone as 10157-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas slide using 10157-1-AP (NPR2L,TUSC4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 10157-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas using 10157-1-AP (same clone as 10157-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas slide using 10157-1-AP (NPR2L,TUSC4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 10157-1-PBS in a different storage buffer formulation.
IHC staining of human skeletal muscle using 10157-1-AP (same clone as 10157-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 10157-1-AP (NPR2L,TUSC4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 10157-1-PBS in a different storage buffer formulation.
IHC staining of human skeletal muscle using 10157-1-AP (same clone as 10157-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 10157-1-AP (NPR2L,TUSC4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 10157-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

NPRL2 Polyclonal antibody, PBS Only

NPRL2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 10157-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

10157-1-PBS targets NPRL2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0210 Product name: Recombinant human NPR2L,TUSC4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-270 aa of BC002791 Sequence: GCRIECIFFSEFHPTLGPKITYQVPEDFISRELFDTVQVYIITKPELQNKLITVTAMEKKLIGCPVCIEHKKYSRNALLFNLGFVCDAQAKTCALEPIVKKLAGYLTTLELESSFVSMEESKQKLVPIMTILLEELNASGRCTLPIDESNTIHLKVIEQRPDPPVAQEYDVPVFTKDKEDFFNSQWDLTTQQILPYIDGFRHIQKISAEADVELNLVRIAIQNLLYYGVVTLVSILQYSNVYCPTPKVQDLVDDKSLQEACLSYVTKQGH Predict reactive species
Full Name	tumor suppressor candidate 4
Calculated Molecular Weight	44 kDa
Observed Molecular Weight	44-50 kDa
GenBank Accession Number	BC002791
Gene Symbol	TUSC4
Gene ID (NCBI)	10641
RRID	AB_514025
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8WTW4
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

NPRL2 (NPR2 Like, GATOR1 Complex Subunit) is a Protein Coding gene associated with Epilepsy, Familial Focal. As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. Homologous to yeast nitrogen permease (candidate tumor suppressor) (NM_006545, BC021984) gene encodes a protein of molecular mass of 50 kDa. Data by using overlapping and nested homozygous deletions methods indicate that this protein might be a tumor suppressor involved in the development of lung cancers.