Tested Applications
| Positive WB detected in | A549 cells, HepG2 cells, mouse skeletal muscle tissue, Jurkat cells, NIH/3T3 cells, Y79 cells, HeLa cells, mouse heart tissue, rat heart tissue |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14506-1-AP targets NR1D1 in WB, IHC, IF/ICC, IP, chip, ELISA, CUT&Tag applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, hamster, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5964 Product name: Recombinant human NR1D1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC056148 Sequence: MTTLDSNNNTGGVITYIGSSGSSPSRTSPESLYSDNSNGSFQSLTQGCPTYFPPSPTGSLTQDPARSFGSIPPSLSDDGSPSSSSSSSSSSSSFYNGSPPGSLQVAMEDSSRVSPSKSTSNITKLNGMVLLCKVCGDVASGFHYGVHACEGCKGFFRRSIQQNIQYKRCLKNENCSIVRINRNRCQQCRFKKCLSVGMSRDAVRFGRIPKREKQRMLAEMQSAMNLANNQLSSQCPLETSPTQHPTPGPMGPSPPPAPVPSPLVGFSQFPQQLTPPRSPSPEPTVEDVISQVARAHREIFTYAHDKLGSSPGNFNANHASGSPPATTPHRWENQGCPPAPNDNNTLAAQR Predict reactive species |
| Full Name | nuclear receptor subfamily 1, group D, member 1 |
| Calculated Molecular Weight | 67 kDa |
| Observed Molecular Weight | 68 kDa |
| GenBank Accession Number | BC056148 |
| Gene Symbol | NR1D1 |
| Gene ID (NCBI) | 9572 |
| RRID | AB_11182604 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P20393 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NR1D1, also named EAR1, THRAL, ear-1, and hRev, contains one nuclear receptor DNA-binding domain and belongs to the nuclear hormone receptor family. NR1 subfamily. This protein functions as a constitutive transcriptional repressor. It is a possible receptor for triiodothyronine. BMAL1-CLOCK activates Rev-erba itself and thereby represents the link between the positive and negative loops of the circadian clock. This is a rabbit polyclonal antibody raised against the N terminus of human NR1D1.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NR1D1 antibody 14506-1-AP | Download protocol |
| IHC protocol for NR1D1 antibody 14506-1-AP | Download protocol |
| WB protocol for NR1D1 antibody 14506-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-NR1D1 (REV-ERBα) antibody (Cat# 14506-1-AP) has 53 citations published in journals including Nature Communications, Cell Metabolism, JCI, Cancer Research, PNAS, Diabetologia, and Theranostics. Research fields span circadian biology, hepatic and lipid metabolism, neurology (Parkinson's, epilepsy, depression), cancer, fibrosis, inflammation, immunology, diabetes, and reproductive biology. Applications include WB, ChIP, IF, IHC, and IP across human, mouse, rat, and other species.
| Species | Application | Title |
|---|---|---|
Cell Metab Intestinal clock shapes sleep-wake cycle via sustaining glutamine homeostasis. | ||
Cancer Res NR1D1 recruitment to sites of DNA damage inhibits repair and is associated with chemosensitivity of breast cancer. | ||
Nat Commun The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock. | ||
Nat Commun Dysregulation of REV-ERBα impairs GABAergic function and promotes epileptic seizures in preclinical models | ||
Metabolism LncRNA Nron deficiency protects mice from diet-induced adiposity and hepatic steatosis | ||
Theranostics PPAR-γ integrates obesity and adipocyte clock through epigenetic regulation of Bmal1. |
Reviews
What customers say
Both reviewers rated this antibody 5 stars for Western Blot at a 1:1000 dilution. One user successfully applied it to cardiomyocytes, mouse skin, and liver tissues. The other observed clear bands in INS1 cells, detecting a band around 67 kDa along with a second, lower-molecular-weight band. Overall, users report reliable performance and clear detection across multiple cell types and tissues.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Priya (Verified Customer) (01-17-2023) | I have used for cardiomyocytes, mouse skin and liver tissues
|
Satish (Verified Customer) (12-19-2019) | I got clear bands. The antibody detects two band one band around 67 kDa and another band much lower than 67kDa.
|
