NR1I3 Polyclonal antibody

• Featured Product
• KD/KO Validated

NR1I3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (3)

Cat no : 21042-1-AP

Synonyms

CAR, CAR BETA, CAR1, Constitutive active response, MB67, NR1I3, Orphan nuclear receptor MB67

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Validation Data Gallery

• 

  WB analysis of HeLa using 21042-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21042-1-AP (NR1I3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 21042-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21042-1-AP (NR1I3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 21042-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21042-1-AP (NR1I3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 21042-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21042-1-AP (NR1I3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 21042-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21042-1-AP (NR1I3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 21042-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 21042-1-AP (NR1I3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 21042-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 21042-1-AP (NR1I3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, L02 cells
• Positive IHC detected in: mouse kidney tissue, rat liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 4 publications below

Product Information

21042-1-AP targets NR1I3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NR1I3 fusion protein Ag15378
• Full Name: nuclear receptor subfamily 1, group I, member 3
• Calculated Molecular Weight: 352 aa, 40 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC069651
• Gene Symbol: NR1I3
• Gene ID (NCBI): 9970
• RRID: AB_10732602
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The nuclear hormone receptor superfamily is a large group of related transcription factors which includes members that bind a diverse array of ligands, including steroids, retinoic acid, thyroid hormone, and vitamin D. Nuclear hormone receptors contain a DNA-binding domain (DBD) and a ligand-binding domain [PMID: 10221899]. Nuclear receptor subfamily 1 group I member 3 (NR1I3; CAR), binds and transactivates the retinoic acid response elements that control expression of the retinoic acid receptor beta 2 and alcohol dehydrogenase 3 genes. It is expressed primarily in liver and regulates the expression of genes involved in xenobiotic metabolism as well as hormone, energy, and lipid homeostasis [PMID:22896671].

Publications

Species	Application	Title
human	WB	Biochem Pharmacol; Constitutive androstane receptor weakens the induction of panaxytriol on CYP3A4 by repressing the activation of pregnane X receptor.; Authors - Qingqing Hu ; KD Validated
human	WB	Front Pharmacol; Oleanolic Acid and Ursolic Acid Induce UGT1A1 Expression in HepG2 Cells by Activating PXR Rather Than CAR.; Authors - Na Yao ; KD Validated
human	WB	Xenobiotica; A high-throughput cell-based gaussia luciferase reporter assay for measurement of CYP1A1, CYP2B6, and CYP3A4 induction.; Authors - Han Li
human	WB	J Ethnopharmacol; Panaxytriol upregulates CYP3A4 expression through the interaction between nuclear regulators and DNA response elements; Authors - Jingdi Yan ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kamal (Verified Customer) (02-15-2024)

This antibody never lets me down. Purchased again for immunohistochemistry.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse liver tissue

FH

Kamal (Verified Customer) (07-05-2023)

Worked well when diluted in TBST (1:2000) for WB and 1:200 in 1X PBS for IFC.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:2000 (WB) and 1:200 (IFC)
• Cell Tissue Type: Mouse Liver tissue

FH

Zehua (Verified Customer) (11-07-2019)

It works well for immunoprecipitation!

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HepG2 cell

NR1I3 Antibody Western Blot,Immunoprecipitation, validation (1:1000 dilution) in HepG2 cell (Cat no:21042-1-AP)