Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 5 publications below |
Product Information
21042-1-AP targets CAR/NR1I3 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15378 Product name: Recombinant human NR1I3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 7-156 aa of BC069651 Sequence: RRTVSKSIGPTCPFAGSCEVSKTQRRHCPACRLQKCLDAGMRKDMILSAEALALRRAKQAQRRAQQTPVQLSKEQEELIRTLLGAHTRHMGTMFEQFVQFRPPAHLFIHHQPLPTLAPVLPLVTHFADINTFMVLQVIKFTKDLPVFRSL Predict reactive species |
| Full Name | nuclear receptor subfamily 1, group I, member 3 |
| Calculated Molecular Weight | 352 aa, 40 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC069651 |
| Gene Symbol | NR1I3 |
| Gene ID (NCBI) | 9970 |
| RRID | AB_10732602 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14994 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The nuclear hormone receptor superfamily is a large group of related transcription factors which includes members that bind a diverse array of ligands, including steroids, retinoic acid, thyroid hormone, and vitamin D. Nuclear hormone receptors contain a DNA-binding domain (DBD) and a ligand-binding domain [PMID: 10221899]. Nuclear receptor subfamily 1 group I member 3 (NR1I3; Constitutive androstane receptor: CAR), binds and transactivates the retinoic acid response elements that control expression of the retinoic acid receptor beta 2 and alcohol dehydrogenase 3 genes. It is expressed primarily in liver and regulates the expression of genes involved in xenobiotic metabolism as well as hormone, energy, and lipid homeostasis [PMID:22896671].
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CAR/NR1I3 antibody 21042-1-AP | Download protocol |
| WB protocol for CAR/NR1I3 antibody 21042-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biochem Pharmacol Constitutive androstane receptor weakens the induction of panaxytriol on CYP3A4 by repressing the activation of pregnane X receptor.
| ||
Front Pharmacol Oleanolic Acid and Ursolic Acid Induce UGT1A1 Expression in HepG2 Cells by Activating PXR Rather Than CAR.
| ||
Xenobiotica A high-throughput cell-based gaussia luciferase reporter assay for measurement of CYP1A1, CYP2B6, and CYP3A4 induction. | ||
J Ethnopharmacol Panaxytriol upregulates CYP3A4 expression through the interaction between nuclear regulators and DNA response elements
| ||
PLoS Pathog Lactobacillus gasseri ATCC33323 affects the intestinal mucosal barrier to ameliorate DSS-induced colitis through the NR1I3-mediated regulation of E-cadherin |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Kamal (Verified Customer) (02-15-2024) | This antibody never lets me down. Purchased again for immunohistochemistry.
|
FH Kamal (Verified Customer) (07-05-2023) | Worked well when diluted in TBST (1:2000) for WB and 1:200 in 1X PBS for IFC.
|
FH Zehua (Verified Customer) (11-07-2019) | It works well for immunoprecipitation!
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