Nurr1/NR4A2 Monoclonal antibody

Nurr1/NR4A2 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1H5B4

Cat no : 66878-1-Ig

Synonyms

HZF 3, NOT, NR4A2, NURR1, Orphan nuclear receptor NURR1, RNR1, TINUR



Tested Applications

Positive WB detected inJurkat cells, Ramos cells, Daudi cells, HT-29 cells, Raji cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

66878-1-Ig targets Nurr1/NR4A2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Nurr1/NR4A2 fusion protein Ag1418
Full Name nuclear receptor subfamily 4, group A, member 2
Calculated molecular weight 66 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC009288
Gene symbol NR4A2
Gene ID (NCBI) 4929
RRIDAB_2882211
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NR4A2 belongs to the orphan nuclear receptor (NR) family referred to as NR4A family, which have been implicated in cell cycle regulation, apoptosis, inflammation, metabolism and more recently in carcinogenesis [PMID:22583411]. NR4A2 also has a role in the expression of several proteins that are necessary for the synthesis and regulation of DA, such as tyrosine hidroxilase, DA transporter, vesicular monoamine transporter 2, and cRET [PMID:22294735]. Act as a transcriptional regulator, NR4A2 is essential for the differentiation and maintenance of meso-diencephalic mdDA neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons [PMID: 17184956].

Protocols

Product Specific Protocols
WB protocol for Nurr1/NR4A2 antibody 66878-1-IgDownload protocol
IHC protocol for Nurr1/NR4A2 antibody 66878-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Mol Biosci

Single-Cell Profiles of Age-Related Osteoarthritis Uncover Underlying Heterogeneity Associated With Disease Progression.

Authors - Wenzhou Liu
  • KD Validated
ratWB,IHC

Mol Cell Neurosci

BDNF-modified human umbilical cord mesenchymal stem cells-derived dopaminergic-like neurons improve rotation behavior of Parkinson's disease rats through neuroprotection and anti-neuroinflammation

Authors - Zhi Jiang
mouseWB

World J Gastroenterol

3,6-dichlorobenzo[b]thiophene-2-carboxylic acid alleviates ulcerative colitis by suppressing mammalian target of rapamycin complex 1 activation and regulating intestinal microbiota

Authors - Qiong-Zi He
human,ratWB

J Pers Med

Identification and Validation of Hub Genes in the Stenosis of Arteriovenous Fistula

Authors - Yu Li