NRD1 Polyclonal antibody

NRD1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 15630-1-AP

Synonyms

hNRD1, hNRD2, N arginine dibasic convertase, Nardilysin, NRD C, NRD convertase, NRD1



Tested Applications

Positive WB detected inK-562 cells
Positive IHC detected inmouse testis tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15630-1-AP targets NRD1 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NRD1 fusion protein Ag7923
Full Name nardilysin (N-arginine dibasic convertase)
Calculated molecular weight 132 kDa
Observed molecular weight 135-140 kDa
GenBank accession numberBC008775
Gene symbol NRD1
Gene ID (NCBI) 4898
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NRD1(Nardilysin) is also named as NRD-C(N-arginine dibasic convertase). It is a zinc metalloendopeptidase of the M16 pitrilysin family that cleaves peptides at dibasic residues. The enzyme has also been found in neural tissues of mouse embryos indicating a role in neural development. Although nardilysin is primarily found in the cytosol, it has been reported that nardilysin is located on the cell surface acting as a receptor for heparinbinding EGF-like growth factor(PMID:15809022). It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for NRD1 antibody 15630-1-APDownload protocol
IHC protocol for NRD1 antibody 15630-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Immunol

Loss of Peripheral Protection in Pancreatic Islets by Proteolysis-Driven Impairment of VTCN1 (B7-H4) Presentation Is Associated with the Development of Autoimmune Diabetes.

Authors - Ilian A Radichev
mouseIF

Diabetes

Nardilysin-dependent proteolysis of cell-associated VTCN1 (B7-H4) marks type 1 diabetes development.

Authors - Ilian A Radichev