NUP214 Recombinant monoclonal antibody, PBS Only (Detector)

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  Sandwich ELISA standard curve of MP01882-3

  Sandwich ELISA standard curve of MP01882-3, Human NUP214 Recombinant Matched Antibody Pair - PBS only. 85161-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag21377. 85161-5-PBS was HRP conjugated as the detection antibody. Range: 1.56-100 ng/mL.

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  Sample test of MP01882-3

  The mean NUP214 concentration was determined to be 133.70 ng/mL in K-562 cell extract based on a 2.50 mg/mL extract load and 153.72 ng/mL in HeLa cell extract based on a 1.50 mg/mL extract load.

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  Affinity and Kinetic Characterization of 85161-5-PBS

  Biolayer interferometry (BLl) kinetic assay of 85161-5-PBS against Human NUP214 was performed. The affinity constant is below 1 pM.

NUP214 Uni-rAb^® Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 85161-5-PBS

Clone No.242782G10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

CAIN, CAN, KIAA0023

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Product Information

85161-5-PBS targets NUP214 as part of a matched antibody pair:

MP01882-3: 85161-2-PBS capture and 85161-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag21377

  Product name: Recombinant human NUP214 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1-350 aa of BC105998

  Sequence: MGDEMDAMIPEREMKDFQFRALKKVRIFDSPEELPKERSSLLAVSNKYGLVFAGGASGLQIFPTKNLLIQNKPGDDPNKIVDKVQGLLVPMKFPIHHLALSCDNLTLSACMMSSEYGSIIAFFDVRTFSNEAKQQKRPFAYHKLLKDAGGMVIDMKWNPTVPSMVAVCLADGSIAVLQVTETVKVCATLPSTVAVTSVCWSPKGKQLAVGKQNGTVVQYLPTLQEKKVIPCPPFYESDHPVRVLDVLWIGTYVFAIVYAAADGTLETSPDVVMALLPKKEEKHPEIFVNFMEPCYGSCTERQHHYYLSYIEEWDLVLAASAASTEVSILARQSDQINWESWLLEDSSRAE

• Full Name: nucleoporin 214kDa
• Calculated Molecular Weight: 2080 aa, 213 kDa
• GenBank Accession Number: BC105998
• Gene Symbol: NUP214
• Gene ID (NCBI): 8021
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P35658
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.