NUP54 Polyclonal antibody

NUP54 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 16232-1-AP

Synonyms

Nucleoporin p54, nucleoporin 54kDa, 54 kDa nucleoporin

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Validation Data Gallery

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  WB analysis of HeLa using 16232-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 16232-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16232-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 16232-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 16232-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16232-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 16232-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16232-1-AP (NUP54 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16232-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16232-1-AP (NUP54 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells, mouse liver tissue, HepG2 cells, mouse heart tissue, A549 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:300-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below

Product Information

16232-1-AP targets NUP54 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NUP54 fusion protein Ag8882
• Full Name: nucleoporin 54kDa
• Calculated Molecular Weight: 507 aa, 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC012559
• Gene Symbol: NUP54
• Gene ID (NCBI): 53371
• RRID: AB_10644484
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Elife; Nuclear pore heterogeneity influences HIV-1 infection and the antiviral activity of MX2.; Authors - Melissa Kane
human	WB	J Exp Clin Cancer Res; Transketolase (TKT) activity and nuclear localization promote hepatocellular carcinoma in a metabolic and a non-metabolic manner.; Authors - Zhaoyu Qin
mouse	WB	Cell Rep; Nucleoporin Seh1 maintains Schwann cell homeostasis by regulating genome stability and necroptosis; Authors - Mei Wu
		Dev Cell; Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination; Authors - Li Li
human	WB	bioRxiv; Global mapping of the Chlamydia trachomatis conventional secreted effector - host interactome reveals CebN interacts with nucleoporins and Rae1 to impede STAT1 nuclear translocation; Authors - Brianna Steiert
human	WB	Nat Phys; Structural anisotropy results in mechano-directional transport of proteins across nuclear pores; Authors - Fani Panagaki ; KD Validated