NUP62CL Polyclonal antibody

NUP62CL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25381-1-AP

Synonyms

NUP62CL, NUP62L

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Validation Data Gallery

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  WB analysis of HeLa using 25381-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25381-1-AP (NUP62CL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 25381-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25381-1-AP (NUP62CL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 25381-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25381-1-AP (NUP62CL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 25381-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25381-1-AP (NUP62CL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, SH-SY5Y cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25381-1-AP targets NUP62CL in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NUP62CL fusion protein Ag21107
• Full Name: nucleoporin 62kDa C-terminal like
• Calculated Molecular Weight: 72 aa, 8 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC017799
• Gene Symbol: NUP62CL
• Gene ID (NCBI): 54830
• RRID: AB_2880051
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.