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Tested Applications
| Positive WB detected in | mouse brain tissue, mouse pancreas tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
15986-1-AP targets Nogo B receptor in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Nogo B receptor fusion protein Ag8789 Predict reactive species |
| Full Name | nuclear undecaprenyl pyrophosphate synthase 1 homolog (S. cerevisiae) |
| Calculated Molecular Weight | 293 aa, 33 kDa |
| Observed Molecular Weight | 30-33 kDa |
| GenBank Accession Number | BC013026 |
| Gene Symbol | Nogo B receptor |
| Gene ID (NCBI) | 116150 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96E22 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Nogo proteins containing three isoforms (Nogo A, B, C) have been implicated in a variety of critical cellular processes including CNS neuronal regeneration, vascular cell spreading and chemotaxis. While only two functional receptors have been reported so far: NgR binding all three Nogos and NgBR specifically binding Nogo-B. Nogo-B receptor, also known as NgBR or NUS1, specifically binds the amino amino terminus (residues 1-200) of Nogo-B, the interaction of which is essential for stimulating chemotaxis, morphogenesis and angiogenesis.Western blot analysis detected high NgBR expression in mouse heart, liver, kidney, and pancreas with molecular weight around 30-33 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Nogo B receptor antibody 15986-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
