NXN antibody (16128-1-PBS)

Filter: AllWBIPIHCIF/ICC

WB analysis of HeLa using 16128-1-AP (same clone as 16128-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 16128-1-AP (NXN antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.
WB analysis using 16128-1-AP (same clone as 16128-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 16128-1-AP (NXN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.
WB analysis of human heart using 16128-1-AP (same clone as 16128-1-PBS)
human heart tissue were subjected to SDS PAGE followed by western blot with 16128-1-AP (NXN antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 16128-1-AP (same clone as 16128-1-PBS)
IP result of anti-NXN (IP:16128-1-AP, 4ug; Detection:16128-1-AP 1:500) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 16128-1-AP (same clone as 16128-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16128-1-AP (NXN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 16128-1-AP (same clone as 16128-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16128-1-AP (NXN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 16128-1-AP (same clone as 16128-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NXN antibody (16128-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.

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Tested Applications
Product Information

Product Information

16128-1-PBS targets NXN in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NXN fusion protein Ag9113 Predict reactive species
Full Name	nucleoredoxin
Calculated Molecular Weight	435 aa, 48 kDa
Observed Molecular Weight	48 kDa
GenBank Accession Number	BC009327
Gene Symbol	NXN
Gene ID (NCBI)	64359
RRID	AB_2158114
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6DKJ4
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Nucleoredoxin (NRX/NXN), a relatively uncharacterized member of the TRX family protein, has recently been reported to regulate the Wnt/β-catenin pathway, which itself regulates cell fate and early development, in a redox-dependent manner(PMID:17567240). Northern blot analysis detected several nucleoredoxin splice variants in adult mouse tissues, with highest expression in testis and skin and lowest expression in liver(PMID:9119370). It has 3 isoforms produced by alternative splicing. This antibody is specific to NXN.