NXN Polyclonal antibody, PBS Only

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  WB analysis of HeLa using 16128-1-AP (same clone as 16128-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16128-1-AP (NXN antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 16128-1-AP (same clone as 16128-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16128-1-AP (NXN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 16128-1-AP (same clone as 16128-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 16128-1-AP (NXN antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 16128-1-AP (same clone as 16128-1-PBS)

  IP result of anti-NXN (IP:16128-1-AP, 4ug; Detection:16128-1-AP 1:500) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 16128-1-AP (same clone as 16128-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16128-1-AP (NXN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 16128-1-AP (same clone as 16128-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16128-1-AP (NXN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 16128-1-AP (same clone as 16128-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NXN antibody (16128-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

NXN Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 16128-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

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Product Information

16128-1-PBS targets NXN in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9113

  Product name: Recombinant human NXN protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-326 aa of BC009327

  Sequence: ADVSLHRNPATLKLWNKYRISNIPSLIFLDATTGKVVCRNGLLVIRDDPEGLEFPWGPKPFREVIAGPLLRNNGQSLESSSLEGSHVGVYFSAHWCPPCRSLTRVLVESYRKIKEAGQNFEIIFVSADRSEESFKQYFSEMPWLAVPYTDEARRSRLNRLYGIQGIPTLIMLDPQGEVITRQGRVEVLNDEDCREFPWHPKPVLELSDSNAAQLNEGPCLVLFVDSEDDGESEAAKQLIQPIAEKIIAKYKAKEEEAPLLFFVAGEDDMTDSLRDYTNLPEAAPLLTILDMSARAKYVMDVEEITPAIVEAFVNDFLAEKLKPEPI

• Full Name: nucleoredoxin
• Calculated Molecular Weight: 435 aa, 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC009327
• Gene Symbol: NXN
• Gene ID (NCBI): 64359
• RRID: AB_2158114
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6DKJ4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Nucleoredoxin (NRX/NXN), a relatively uncharacterized member of the TRX family protein, has recently been reported to regulate the Wnt/β-catenin pathway, which itself regulates cell fate and early development, in a redox-dependent manner(PMID:17567240). Northern blot analysis detected several nucleoredoxin splice variants in adult mouse tissues, with highest expression in testis and skin and lowest expression in liver(PMID:9119370). It has 3 isoforms produced by alternative splicing. This antibody is specific to NXN.