|Positive WB detected in||HeLa cells, human heart tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
16128-1-AP targets NXN in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NXN fusion protein Ag9113|
|Calculated molecular weight||435 aa, 48 kDa|
|Observed molecular weight||48 kDa|
|GenBank accession number||BC009327|
|Gene ID (NCBI)||64359|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Nucleoredoxin (NRX/NXN), a relatively uncharacterized member of the TRX family protein, has recently been reported to regulate the Wnt/β-catenin pathway, which itself regulates cell fate and early development, in a redox-dependent manner(PMID:17567240). Northern blot analysis detected several nucleoredoxin splice variants in adult mouse tissues, with highest expression in testis and skin and lowest expression in liver(PMID:9119370). It has 3 isoforms produced by alternative splicing. This antibody is specific to NXN.
|Product Specific Protocols|
|WB protocol for NXN antibody 16128-1-AP||Download protocol|
|IHC protocol for NXN antibody 16128-1-AP||Download protocol|
|IF protocol for NXN antibody 16128-1-AP||Download protocol|
|IP protocol for NXN antibody 16128-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Oxid Med Cell Longev
Nucleoredoxin-Dependent Targets and Processes in Neuronal Cells.
A crucial role for Nox 1 in redox-dependent regulation of Wnt-β-catenin signaling.
Mitochondrial reactive oxygen species promote epidermal differentiation and hair follicle development.