NXN Polyclonal antibody

NXN Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 16128-1-AP

Synonyms

NRX, nucleoredoxin, NXN, TRG 4



Tested Applications

Positive WB detected inHeLa cells, human heart tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

16128-1-AP targets NXN in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NXN fusion protein Ag9113
Full Name nucleoredoxin
Calculated molecular weight 435 aa, 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC009327
Gene symbol NXN
Gene ID (NCBI) 64359
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nucleoredoxin (NRX/NXN), a relatively uncharacterized member of the TRX family protein, has recently been reported to regulate the Wnt/β-catenin pathway, which itself regulates cell fate and early development, in a redox-dependent manner(PMID:17567240). Northern blot analysis detected several nucleoredoxin splice variants in adult mouse tissues, with highest expression in testis and skin and lowest expression in liver(PMID:9119370). It has 3 isoforms produced by alternative splicing. This antibody is specific to NXN.

Protocols

Product Specific Protocols
WB protocol for NXN antibody 16128-1-APDownload protocol
IHC protocol for NXN antibody 16128-1-APDownload protocol
IF protocol for NXN antibody 16128-1-APDownload protocol
IP protocol for NXN antibody 16128-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oxid Med Cell Longev

Nucleoredoxin-Dependent Targets and Processes in Neuronal Cells.

Authors - Claudia Urbainsky
human,mouse,ratWB

FASEB J

A crucial role for Nox 1 in redox-dependent regulation of Wnt-β-catenin signaling.

Authors - Kajla Seheli S
mouseWB

Sci Signal

Mitochondrial reactive oxygen species promote epidermal differentiation and hair follicle development.

Authors - Hamanaka Robert B RB