Product Information
85299-5-PBS targets Nidogen 2 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag4643 Product name: Recombinant human NID2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-300 aa of BC035608 Sequence: MEGDRVAGRPVLSSLPVLLLLQLLMLRAAALHPDELFPHGESWGDQLLQEGDDESSAVVKLANPLHFYEARFSNLYVGTNGIISTQDFPRETQYVDYDFPTDFPAIAPFLADIDTSHGRGRVLYRVNGKVSGHLHVGHTPVHFTDVDLHAYIVGNDGRAYTAISHIPQPAAQALLPLTPIGGLFGWLFALEKPGSENGFSLAGAAFTHDMEVTFYPGEETVRITQTAEGLDPENYLSIKTNIQGQVPYVPANFTAHISPYKELYHYSDSTVTSTSSRDYSLTFGAINQTWSYRIHQNITY Predict reactive species |
| Full Name | nidogen 2 (osteonidogen) |
| Calculated Molecular Weight | 1375 aa, 151 kDa |
| Observed Molecular Weight | 200 kDa |
| GenBank Accession Number | BC035608 |
| Gene Symbol | NID2 |
| Gene ID (NCBI) | 22795 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q14112 |
| Storage Buffer | PBS only, pH 7.3. |
| Storage Conditions | Store at -80°C. |
Background Information
Nidogen, also referred to as entactin, represents a highly conserved protein in vertebrates and invertebrates and is found in almost all basement membranes (BM). Nidogen-1 (150 kDa) and nidogen-2 (200 kDa) are elongated molecules composed of three globular domains (G1, G2, and G3) connected by a flexible, protease-sensitive link and a rigid rod-like domain. Western blot analysis of protein extracts of heart, lung, and skeletal muscle of mice, major bands of about 200 kDa were detected, accompanied by smaller and variable bands of 160-170 kDa. (PMID: 12215539)
