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  • KD/KO Validated

OGDHL Polyclonal antibody

OGDHL Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17110-1-AP

Synonyms

KIAA1290, OGDC E1 like, OGDHL



Tested Applications

Positive WB detected inmouse kidney tissue, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

17110-1-AP targets OGDHL in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OGDHL fusion protein Ag10794
Full Name oxoglutarate dehydrogenase-like
Calculated molecular weight 1010 aa, 115 kDa
Observed molecular weight 115 kDa
GenBank accession numberBC026320
Gene symbol OGDHL
Gene ID (NCBI) 55753
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

OGDHL(oxoglutarate dehydrogenase-like) is also named as KGDE1KL, FLJ10851, KIAA1290 and belongs to the alpha-ketoglutarate dehydrogenase family. OGDHL is one of the rate-limiting component of the multienzyme OGDH complex (OGDHC) whose malfunction is associated with neuro-degeneration and it is localized only in the mitochondrial fraction and not in the cytosolic or nuclear fractions(PMID:23152800). OGDHL negatively regulates cell proliferation by inducing apoptosis in cervical cancer cells. It may thus participate in brain-specific control of 2-oxoglutarate distribution between energy production and synthesis of the neurotransmitter glutamate(PMID:18783430). It has 3 isoforms(114 kDa, 108 kDa, 91 kDa) produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for OGDHL antibody 17110-1-APDownload protocol
IHC protocol for OGDHL antibody 17110-1-APDownload protocol
IP protocol for OGDHL antibody 17110-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Hepatol

OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.

Authors - Weiqi Dai
  • KD Validated
humanWB

Am J Hum Genet

Bi-allelic variants in OGDHL cause a neurodevelopmental spectrum disease featuring epilepsy, hearing loss, visual impairment, and ataxia.

Authors - Zheng Yie Yap
mouseWB

Front Cell Dev Biol

Cardioprotective Role of SIRT5 in Response to Acute Ischemia Through a Novel Liver-Cardiac Crosstalk Mechanism.

Authors - Boda Zhou
ratWB

Transl Psychiatry

Hippocampal proteomic changes of susceptibility and resilience to depression or anxiety in a rat model of chronic mild stress.

Authors - Min Tang
humanIHC

Front Cardiovasc Med

Osteopontin and LDLR Are Upregulated in Hearts of Sudden Cardiac Death Victims With Heart Failure With Preserved Ejection Fraction and Diabetes Mellitus.

Authors - Mausam Patel
humanIF

PLoS One

OGDHL is a modifier of AKT-dependent signaling and NF-κB function.

Authors - Sen Tanusree T