|Positive WB detected in||mouse kidney tissue, mouse brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
17110-1-AP targets OGDHL in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||OGDHL fusion protein Ag10794|
|Full Name||oxoglutarate dehydrogenase-like|
|Calculated molecular weight||1010 aa, 115 kDa|
|Observed molecular weight||115 kDa|
|GenBank accession number||BC026320|
|Gene ID (NCBI)||55753|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
OGDHL(oxoglutarate dehydrogenase-like) is also named as KGDE1KL, FLJ10851, KIAA1290 and belongs to the alpha-ketoglutarate dehydrogenase family. OGDHL is one of the rate-limiting component of the multienzyme OGDH complex (OGDHC) whose malfunction is associated with neuro-degeneration and it is localized only in the mitochondrial fraction and not in the cytosolic or nuclear fractions(PMID:23152800). OGDHL negatively regulates cell proliferation by inducing apoptosis in cervical cancer cells. It may thus participate in brain-specific control of 2-oxoglutarate distribution between energy production and synthesis of the neurotransmitter glutamate(PMID:18783430). It has 3 isoforms(114 kDa, 108 kDa, 91 kDa) produced by alternative splicing.
OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.
Am J Hum Genet
Bi-allelic variants in OGDHL cause a neurodevelopmental spectrum disease featuring epilepsy, hearing loss, visual impairment, and ataxia.
Front Cell Dev Biol
Cardioprotective Role of SIRT5 in Response to Acute Ischemia Through a Novel Liver-Cardiac Crosstalk Mechanism.
Hippocampal proteomic changes of susceptibility and resilience to depression or anxiety in a rat model of chronic mild stress.
Front Cardiovasc Med
Osteopontin and LDLR Are Upregulated in Hearts of Sudden Cardiac Death Victims With Heart Failure With Preserved Ejection Fraction and Diabetes Mellitus.
OGDHL is a modifier of AKT-dependent signaling and NF-κB function.