hIST1 Polyclonal antibody

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  WB analysis using 51002-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51002-1-AP (hIST1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 51002-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51002-1-AP (hIST1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 51002-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51002-1-AP (hIST1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

hIST1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 51002-1-AP

Publications(26)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, CoIP, ELISA

Synonyms

hIST1, IST1, IST1 homolog, KIAA0174, OLC1

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, PC-3 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 12 publications below
• IHC: See 3 publications below
• IF: See 15 publications below
• CoIP: See 1 publications below

Product Information

51002-1-AP targets hIST1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0721

  Product name: Recombinant human hIST1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-360 aa of BC000430

  Sequence: MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEYGKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEPDSVVMAEAPPGVETDLIDVGFTDDVKKGGPGRGGSGGFTAPVGGPDGTVPMPMPMPMPMPSANTPFSYPLPKGPSDFNGLPMGTYQAFPNIHPPQIPATPPSYESMTLMLIRISLLHRLLVLDPSQKPLQSFLPDLQITMTTLSYQSCHLCQTHYQLHLLVPAPQHLKTLTLMIFPGGLKS

• Full Name: KIAA0174
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC000430
• Gene Symbol: hIST1
• Gene ID (NCBI): 9798
• RRID: AB_2130516
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P53990
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human increased sodium tolerance-1 (hIST1), encoded by KIAA0174 gene, is a newly reported endosomal sorting complexes required for transport (ESCRT)-III family member. IST1 possesses two different types of microtubule-interacting and transport (MIT) -interacting motifs (MIM1 and MIM2), this is required for its interaction with calpain 7 which is important for their roles in the ESCRT system.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Nature; Nuclear envelope assembly defects link mitotic errors to chromothripsis.; Authors - Shiwei Liu
human	WB, IF	Nature; Spastin and ESCRT-III coordinate mitotic spindle disassembly and nuclear envelope sealing.; Authors - Marina Vietri ; KD Validated
human	WB	J Cell Biol; An ESCRT-spastin interaction promotes fission of recycling tubules from the endosome.; Authors - Allison Rachel R ; KD Validated
human	IF	Am J Hum Genet; De Novo VPS4A Mutations Cause Multisystem Disease with Abnormal Neurodevelopment.; Authors - Catherine Rodger
human	IF	J Cell Biol; ALIX and ESCRT-I/II function as parallel ESCRT-III recruiters in cytokinetic abscission.; Authors - Liliane Christ
human	IF	Proc Natl Acad Sci U S A; ESCRT-III binding protein MITD1 is involved in cytokinesis and has an unanticipated PLD fold that binds membranes.; Authors - Hadders Michael A MA

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Stephen (Verified Customer) (04-08-2019)

IF image of Methanol fixed a dividing HeLa cell Stained with IST antibody (green) and Tubulin Antibody (red)

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: HeLa

hIST1 Antibody Immunofluorescence, validation (1:300 dilution) in HeLa (Cat no:51002-1-AP)