OTOF Polyclonal antibody

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• OTOF Polyclonal antibody (20721-1-AP)

OTOF Polyclonal Antibody for IHC, ELISA

Cat No. 20721-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Synonyms

DFNB6, DFNB9, Fer 1 like protein 2, FER1L2, NSRD9, OTOF, otoferlin

• 

  IHC staining of rat brain using 20721-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20721-1-AP (OTOF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat brain using 20721-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20721-1-AP (OTOF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 20721-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20721-1-AP (OTOF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 20721-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20721-1-AP (OTOF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive IHC detected in: rat brain tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20721-1-AP targets OTOF in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: otoferlin
• Calculated Molecular Weight: 227 kDa
• GenBank Accession Number: NM_004802
• Gene Symbol: OTOF
• Gene ID (NCBI): 9381
• RRID: AB_3085618
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9HC10
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Otoferlin, a C2 domain transmembrane protein, is a major calcium sensor regulating extracellular secretion in mammalian internal hair cells. Deafness caused by mutations in the OTOF gene encoding Otoferlin is a recessive genetic disorder, a sensorineural deafness. It is expressed in the brain, inner hair cells and vestibular hair cells.(PMID: 17055430; PMID: 17229086)

Publications

What published studies show

The OTOF Polyclonal antibody (Cat# 20721-1-AP) has 1 citation to date. It appears in Cancers (Basel) (Impact Factor 4.8), published in 2026. The cited study investigates OTOF's role in promoting clear cell renal cell carcinoma progression and angiogenesis through AKT-dependent HIF/VEGFA signaling, placing the research in the fields of cancer biology, tumor angiogenesis, and intracellular signaling pathways.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Cancers (Basel); OTOF Promotes Clear Cell Renal Cell Carcinoma Progression and Angiogenesis Through AKT-Dependent HIF/VEGFA Signaling.; Authors - Jianhua Wen