OTOF Polyclonal antibody

OTOF Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 20721-1-AP

Synonyms

DFNB6, DFNB9, Fer 1 like protein 2, FER1L2, NSRD9, OTOF, otoferlin

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Validation Data Gallery

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  IHC staining of rat brain using 20721-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20721-1-AP (OTOF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 20721-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20721-1-AP (OTOF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 20721-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20721-1-AP (OTOF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 20721-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20721-1-AP (OTOF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: rat brain tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20721-1-AP targets OTOF in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: otoferlin
• Calculated Molecular Weight: 227 kDa
• GenBank Accession Number: NM_004802
• Gene Symbol: OTOF
• Gene ID (NCBI): 9381
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Otoferlin, a C2 domain transmembrane protein, is a major calcium sensor regulating extracellular secretion in mammalian internal hair cells. Deafness caused by mutations in the OTOF gene encoding Otoferlin is a recessive genetic disorder, a sensorineural deafness. It is expressed in the brain, inner hair cells and vestibular hair cells.(PMID: 17055430; PMID: 17229086)