Anti-Mouse OX40L/TNFSF4 Rabbit Recombinant Antibody

• 

  FC experiment of BALB/c mouse splenocytes using 98381-1-RR

  1x10^6 untreated or Anti-IgM and CD40 treated BALB/c mouse splenocytes were surface stained with 0.25 ug Anti-Mouse OX40 Ligand/TNFSF4 Rabbit RecAb (98381-1-RR, Clone:242227B1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 488 Anti-Mouse CD19 (1D3) (CL488-65290, Clone: 1D3). Cells were not fixed.

• 

  FC experiment of BALB/c mouse splenocytes using 98381-1-RR

  1x10^6 Anti-IgM and CD40 treated BALB/c mouse splenocytes were surface stained with 0.25 ug Anti-Mouse OX40 Ligand/TNFSF4 Rabbit RecAb (98381-1-RR, Clone:242227B1) (red) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse OX40 Ligand/TNFSF4 Rabbit RecAb (98381-1-RR, Clone:242227B1) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

OX40L/TNFSF4 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98381-1-RR

Clone No.242227B1

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

CD252, OX40 ligand, OX40L, Tnfsf4, Tumor necrosis factor ligand superfamily member 4

---

Tested Applications

• Positive FC detected in: Anti-IgM and CD40 treated BALB/c mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98381-1-RR targets OX40L/TNFSF4 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: OX40L/TNFSF4 fusion protein Eg1573
• Full Name: tumor necrosis factor (ligand) superfamily, member 4
• Calculated Molecular Weight: 22kDa
• GenBank Accession Number: NM_009452.2
• Gene Symbol: Tnfsf4
• Gene ID (NCBI): 22164
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P43488
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

The tumour necrosis factor ligand superfamily member 4 gene (TNFSF4, OX40L), which encodes for the T cell costimulatory molecule OX40 ligand, has been identified as a susceptibility gene for the development of systemic lupus erythematosus (SLE). OX40L/TNFSF4/CD252 is a co-stimulatory checkpoint protein expressed by several types of immune and non-immune cells (PMID: 15750594, 19778912). TNFSF4 mRNA is expressed in melanoma cell lines and melanoma samples, including those with low lymphocytic infiltrates, and is not associated with the ulceration status of the primary tumor (PMID: 31501955).