OXCT2 Polyclonal antibody

OXCT2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 17122-1-AP

Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

3 oxoacid CoA transferase 2, 3 oxoacid CoA transferase 2A, FKSG25, OXCT2, SCOT T

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis of mouse testis using 17122-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 17122-1-AP (OXCT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse testis using 17122-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 17122-1-AP (OXCT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 17122-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 17122-1-AP (OXCT2 antibody) at dilution of 1:100 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 17122-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 17122-1-AP (OXCT2 antibody) at dilution of 1:100 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human skin using 17122-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 17122-1-AP (OXCT2 antibody) at dilution of 1:100 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human skin using 17122-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 17122-1-AP (OXCT2 antibody) at dilution of 1:100 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lung using 17122-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 17122-1-AP (OXCT2 antibody) at dilution of 1:100 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lung using 17122-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 17122-1-AP (OXCT2 antibody) at dilution of 1:100 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary using 17122-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 17122-1-AP (OXCT2 antibody) at dilution of 1:100 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary using 17122-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 17122-1-AP (OXCT2 antibody) at dilution of 1:100 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 17122-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17122-1-AP (OXCT2 antibody) at dilution of 1:100 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 17122-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17122-1-AP (OXCT2 antibody) at dilution of 1:100 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	mouse testis tissue
Positive IHC detected in	human testis tissue, human kidney tissue, human lung tissue, human ovary tissue, human skin tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17122-1-AP targets OXCT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10810 Product name: Recombinant human OXCT2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-353 aa of BC030015 Sequence: YGTLVQEGGAPIRYTPDGHLALMSQPREVREFNGDHFLLERAIRADFALVKGWKADRAGNVVFRRSARNFNVPMCKAADVTAVEVEEIVEVGAFPPEDIHVPNIYVDRVIKGQKYEKRIERLTILKEEDGDAGKEEDARTRIIRRAALEFEDGMYANLGIGIPLLASNFISPSMTVHLHSENGILGLGPFPTEDEVDADLINAGKQTVTVLPGGCFFASDDSFAMIRGGHIQLTMLGAMQVSKYGDLANWMIPGKKVKGMGGAMDLVSSQKTRVVVTMQHCTKDNTPKIMEKCTMPLTGKRCVDRIITEKAVFDVHRKKELTLRELWEGLTVDDIKKSTGCAFAVSPNLRPMQ Predict reactive species
Full Name	3-oxoacid CoA transferase 2
Calculated Molecular Weight	517 aa, 56 kDa
Observed Molecular Weight	55 kDa
GenBank Accession Number	BC030015
Gene Symbol	OXCT2
Gene ID (NCBI)	64064
RRID	AB_2157447
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BYC2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for OXCT2 antibody 17122-1-AP	Download protocol
WB protocol for OXCT2 antibody 17122-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
human	WB	Cell Res Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress. Authors - De Huang View Article