Occludin Polyclonal antibody

Occludin Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse , rat and More (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

162

Reviews (1)

Cat no : 27260-1-AP

Synonyms

Occludin, OCLN

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Validation Data Gallery

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  WB analysis using 27260-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27260-1-AP (Occludin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27260-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27260-1-AP (Occludin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 27260-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 27260-1-AP (Occludin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 27260-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 27260-1-AP (Occludin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HUVEC using 27260-1-AP

  IP result of anti-Occludin (IP:27260-1-AP, 4ug; Detection:27260-1-AP 1:300) with HUVEC cells lysate 3600 ug.

• 

  IHC staining of human kidney using 27260-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27260-1-AP (Occludin antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 27260-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27260-1-AP (Occludin antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human breast cancer using 27260-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27260-1-AP (Occludin Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27260-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27260-1-AP (Occludin Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Caco-2 using 27260-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using Occludin antibody (27260-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 27260-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using Occludin antibody (27260-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, HUVEC cells, mouse kidney tissue, mouse liver tissue, rat liver tissue, mouse colon tissue
• Positive IP detected in: HUVEC cells
• Positive IHC detected in: human kidney tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Caco-2 cells, MCF-7 cells

Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:400-1:1600

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 138 publications below
• IHC: See 18 publications below
• IF: See 29 publications below
• FC: See 1 publications below

Product Information

The immunogen of 27260-1-AP is Occludin Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse , rat
• Cited Reactivity: bovine, human, mouse, pig, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Occludin fusion protein Ag26173
• Full Name: occludin
• Calculated molecular weight: 522 aa, 59 kDa
• Observed molecular weight: 59 kDa
• GenBank accession number: BC029886
• Gene symbol: OCLN
• Gene ID (NCBI): 4950
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).

Publications

Species	Application	Title
human	WB	Mol Carcinog; TIMM50 promotes tumor progression via ERK signaling and predicts poor prognosis of non-small cell lung cancer patients.; Authors - Xiupeng Zhang
human	WB	Sci Rep; Anti-breast Cancer Activity of SPG-56 from Sweet Potato in MCF-7 Bearing Mice in Situ through Promoting Apoptosis and Inhibiting Metastasis.; Authors - Zhaoxing Li
bovine	WB	J Appl Toxicol; Pharmacological inhibition of TLR4/NF-κB with TLR4-IN-C34 attenuated microcystin-leucine arginine toxicity in bovine Sertoli cells.; Authors - Elikanah Olusayo Adegoke
mouse	IF,WB	Clin Sci (Lond); Activation of G protein-coupled estrogen receptor protects intestine from ischemia/reperfusion injury in mice by protecting the crypt cell proliferation.; Authors - Shiquan Chai
mouse	WB	Int J Biol Macromol; Modulating the gut microbiota and inflammation is involved in the effect of Bupleurum polysaccharides against diabetic nephropathy in mice.; Authors - Yuchen Feng
rat	WB	Neurochem Res; Ischemia-Reperfusion Injury of Sciatic Nerve in Rats: Protective Role of Combination of Vitamin C with E and Tissue Plasminogen Activator.; Authors - Katerina Apostolopoulou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kushal (Verified Customer) (03-14-2022)

• Applications: Western Blot, Immunoprecipitation, ChIP
• Primary Antibody Dilution: 1.2000
• Cell Tissue Type: Surgical human tissue, mouse tissue, Caco-2 cells

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