Occludin Polyclonal antibody

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  WB analysis using 27260-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27260-1-AP (Occludin antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27260-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27260-1-AP (Occludin antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27260-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27260-1-AP (Occludin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27260-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27260-1-AP (Occludin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27260-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27260-1-AP (Occludin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HUVEC using 27260-1-AP

  IP result of anti-Occludin (IP:27260-1-AP, 4ug; Detection:27260-1-AP 1:300) with HUVEC cells lysate 3600 ug.

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  IHC staining of human colon using 27260-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27260-1-AP (Occludin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 27260-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27260-1-AP (Occludin antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 27260-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27260-1-AP (Occludin antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 27260-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27260-1-AP (Occludin antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 27260-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27260-1-AP (Occludin Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27260-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27260-1-AP (Occludin Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 27260-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using Occludin antibody (27260-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of Caco-2 using 27260-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using Occludin antibody (27260-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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  IF Staining of Caco-2 using 27260-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using Occludin antibody (27260-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 27260-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using Occludin antibody (27260-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Occludin Polyclonal Antibody for WB, IHC, IF/ICC, IF-Fro, IP, ELISA

Cat No. 27260-1-AP

Publications(646)

Reviews (3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF/ICC, IF-Fro, IP, ELISA

Synonyms

OCLN

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Tested Applications

• Positive WB detected in: HUVEC cells, HepG2 cells, mouse kidney tissue, mouse liver tissue, rat liver tissue, mouse brain tissue, mouse colon tissue, rat brain tissue
• Positive IP detected in: HUVEC cells
• Positive IHC detected in: human colon tissue, human breast cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-Fro detected in: mouse brain tissue
• Positive IF/ICC detected in: Caco-2 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:10000
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 507 publications below
• IHC: See 93 publications below
• IF: See 189 publications below
• IP: See 1 publications below

Product Information

27260-1-AP targets Occludin in WB, IHC, IF/ICC, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, rabbit, canine, chicken, bovine, goat, duck
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Occludin fusion protein Ag26173
• Full Name: occludin
• Calculated Molecular Weight: 522 aa, 59 kDa
• Observed Molecular Weight: 59 kDa, 32 kDa, 23-25 kDa
• GenBank Accession Number: BC029886
• Gene Symbol: Occludin
• Gene ID (NCBI): 4950
• RRID: AB_2880820
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q16625
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini, and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).

Publications

Species	Application	Title
mouse	IHC	Cell Metab; Acetate enables metabolic fitness and cognitive performance during sleep disruption; Authors - Qinqin He
mouse	IF	J Clin Invest; A20 regulates lymphocyte adhesion in murine neuroinflammation by restricting endothelial ICOSL expression in the CNS; Authors - Lisa Johann
	IF	Microbiome; Arula-7 powder improves diarrhea and intestinal epithelial tight junction function associated with its regulation of intestinal flora in calves infected with pathogenic Escherichia coli O1; Authors - Hao Chen
pig	WB	Microbiome; Zearalenone disturbs the reproductive-immune axis in pigs: the role of gut microbial metabolites; Authors - Shujin Wang
mouse	IF	Adv Sci (Weinh); Plasma-Activated Solutions Mitigates DSS-Induced Colitis via Restoring Redox Homeostasis and Reversing Microbiota Dysbiosis; Authors - Tuanhe Sun
human	WB	J Extracell Vesicles; Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours.; Authors - Xiaoyu Yu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Iram (Verified Customer) (06-13-2022)

Excellent antibody for immunoblotting

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: colon cancer cells

FH

Balawant (Verified Customer) (05-08-2022)

This antibody I have used and is working great . 1:2000 dilution is working great for the cell line (Colonic epithelialcellr of human).

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Human colonic epithelial cells and mice colon tissue

FH

Kushal (Verified Customer) (03-14-2022)

• Applications: Western Blot, Immunoprecipitation, ChIP
• Primary Antibody Dilution: 1.2000
• Cell Tissue Type: Surgical human tissue, mouse tissue, Caco-2 cells