Validation Data Gallery
|Positive IHC detected in
|mouse testis tissue, human osteosarcoma tissue, human testis tissue, mouse kidney tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|U2OS cells, NIH/3T3 cells
|Positive FC detected in
|IHC : 1:50-1:500
|IF : 1:200-1:800
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
23418-1-AP targets Osteocalcin in IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat, pig, canine
|Host / Isotype
|Rabbit / IgG
|Osteocalcin fusion protein Ag20065
|bone gamma-carboxyglutamate (gla) protein
|Calculated molecular weight
|100 aa, 11 kDa
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Osteocalcin is a small, highly conserved molecule associated with mineralization of bone matrix. Osteocalcin is specifically expressed in osteoblasts and is the most abundant non-collagenous protein in bone. It regulates the dynamics of new bone formation and bone resorption by interaction with vitamin D, and by influencing the differentiation of osteoblasts. Osteocalcin is also involved in the posttranslational targeting of vitamin K-dependent gamma-carboxylation, which controls blood coagulation.
Nanotopography Sequentially Mediates Human Mesenchymal Stem Cell-Derived Small Extracellular Vesicles for Enhancing Osteogenesis.
A nano-conductive osteogenic hydrogel to locally promote calcium influx for electro-inspired bone defect regeneration
Targeting actin-bundling protein L-plastin as an anabolic therapy for bone loss.
HuR-mediated nucleocytoplasmic translocation of HOTAIR relieves its inhibition of osteogenic differentiation and promotes bone formation
Small extracellular vesicle-mediated miR-320e transmission promotes osteogenesis in OPLL by targeting TAK1.
J Clin Invest
SIRT6 protects vascular smooth muscle cells from osteogenic transdifferentiation via Runx2 in chronic kidney disease.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
CHAO (Verified Customer) (03-31-2022)
Weak bands around the correct size
LUNFENG (Verified Customer) (01-27-2020)
Jie (Verified Customer) (01-23-2020)
Not working very well in my western blot
Canzhao (Verified Customer) (01-17-2020)
This antibody worked very well on western blot and immunofluorescence, very specific. I recommend this antibody!