Osteocalcin Polyclonal antibody
Osteocalcin Polyclonal Antibody for IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 23418-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | human osteosarcoma tissue, human testis tissue, mouse kidney tissue, mouse testis tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | NIH/3T3 cells |
Recommended dilution
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 21 publications below |
IHC | See 33 publications below |
IF | See 34 publications below |
Product Information
The immunogen of 23418-1-AP is Osteocalcin Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Osteocalcin fusion protein Ag20065 |
Full Name | bone gamma-carboxyglutamate (gla) protein |
Calculated molecular weight | 100 aa, 11 kDa |
GenBank accession number | BC113432 |
Gene symbol | BGLAP |
Gene ID (NCBI) | 632 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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IHC protocol for Osteocalcin antibody 23418-1-AP | Download protocol |
IF protocol for Osteocalcin antibody 23418-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Colloids Surf B Biointerfaces Bi-layered electrospun nanofibrous membrane with osteogenic and antibacterial properties for guided bone regeneration. | ||
FEBS J Diminished membrane recruitment of Akt is instrumental in alcohol-associated osteopenia via the PTEN/Akt/GSK-3β/β-catenin axis. | ||
Arch Gynecol Obstet Oviductus Ranae protein hydrolyzate prevents menopausal osteoporosis by regulating TGFβ/BMP2 signaling. | ||
Cell Death Differ A novel long noncoding RNA PGC1β-OT1 regulates adipocyte and osteoblast differentiation through antagonizing miR-148a-3p. | ||
J Cell Physiol Reduced PDGF-AA in subchondral bone leads to articular cartilage degeneration after strenuous running. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH LUNFENG (Verified Customer) (01-27-2020) | GOOD
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FH Jie (Verified Customer) (01-23-2020) | Not working very well in my western blot
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FH Canzhao (Verified Customer) (01-17-2020) | This antibody worked very well on western blot and immunofluorescence, very specific. I recommend this antibody!
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