Osteocalcin Polyclonal antibody

Osteocalcin Polyclonal Antibody for FC, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 23418-1-AP

Synonyms

BGLAP, Osteocalcin, BGP, Bone Gla protein, OC, OCN, Osteocalcin, PMF1



Tested Applications

Positive IHC detected inmouse testis tissue, human osteosarcoma tissue, human testis tissue, mouse kidney tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells, NIH/3T3 cells
Positive FC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

23418-1-AP targets Osteocalcin in IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Osteocalcin fusion protein Ag20065
Full Name bone gamma-carboxyglutamate (gla) protein
Calculated molecular weight 100 aa, 11 kDa
GenBank accession numberBC113432
Gene symbol BGLAP
Gene ID (NCBI) 632
RRIDAB_2879275
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Osteocalcin is a small, highly conserved molecule associated with mineralization of bone matrix. Osteocalcin is specifically expressed in osteoblasts and is the most abundant non-collagenous protein in bone. It regulates the dynamics of new bone formation and bone resorption by interaction with vitamin D, and by influencing the differentiation of osteoblasts. Osteocalcin is also involved in the posttranslational targeting of vitamin K-dependent gamma-carboxylation, which controls blood coagulation.

Protocols

Product Specific Protocols
IHC protocol for Osteocalcin antibody 23418-1-APDownload protocol
IF protocol for Osteocalcin antibody 23418-1-APDownload protocol
FC protocol for Osteocalcin antibody 23418-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC,IF

ACS Nano

Nanotopography Sequentially Mediates Human Mesenchymal Stem Cell-Derived Small Extracellular Vesicles for Enhancing Osteogenesis.

Authors - Liang Ma
mouseIF

Biomaterials

A nano-conductive osteogenic hydrogel to locally promote calcium influx for electro-inspired bone defect regeneration

Authors - Congcong Yu
mouseIF

Sci Adv

Targeting actin-bundling protein L-plastin as an anabolic therapy for bone loss.

Authors - Xiaoqun Li
mouseIHC

Bone Res

HuR-mediated nucleocytoplasmic translocation of HOTAIR relieves its inhibition of osteogenic differentiation and promotes bone formation

Authors - Yuheng Li
humanIHC

Nat Commun

Small extracellular vesicle-mediated miR-320e transmission promotes osteogenesis in OPLL by targeting TAK1.

Authors - Chen Xu
mouseIF

J Clin Invest

SIRT6 protects vascular smooth muscle cells from osteogenic transdifferentiation via Runx2 in chronic kidney disease.

Authors - Wenxin Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

CHAO (Verified Customer) (03-31-2022)

Weak bands around the correct size

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse Heart
FH

LUNFENG (Verified Customer) (01-27-2020)

GOOD

  • Applications: Immunofluorescence,
  • Cell Tissue Type: HEART
FH

Jie (Verified Customer) (01-23-2020)

Not working very well in my western blot

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse kidney tissue
FH

Canzhao (Verified Customer) (01-17-2020)

This antibody worked very well on western blot and immunofluorescence, very specific. I recommend this antibody!

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:1000 for WB, 1:100 for IF
  • Cell Tissue Type: Mouse heart and cultured cardiomyocytes.