Osteocalcin/OCN Polyclonal antibody

Osteocalcin/OCN Polyclonal Antibody for IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

205

Reviews (4)

Cat no : 23418-1-AP

Synonyms

BGLAP, Osteocalcin, Gamma-carboxyglutamic acid-containing protein, Bone Gla protein, BGP

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Validation Data Gallery

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  IHC staining of mouse testis using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 23418-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 23418-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 23418-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human osteosarcoma using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue slide using 23418-1-AP (Osteocalcin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human osteosarcoma using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue slide using 23418-1-AP (Osteocalcin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 23418-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23418-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23418-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human osteosarcoma using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma slide using 23418-1-AP (Osteocalcin Antibody) at dilution of 1:50.

• 

  IHC staining of human osteosarcoma using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma slide using 23418-1-AP (Osteocalcin Antibody) at dilution of 1:50.

• 

  IHC staining of human osteosarcoma using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue slide using 23418-1-AP (Osteocalcin Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human osteosarcoma using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue slide using 23418-1-AP (Osteocalcin Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 23418-1-AP (Osteocalcin Antibody) at dilution of 1:50.

• 

  IHC staining of human testis using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 23418-1-AP (Osteocalcin Antibody) at dilution of 1:50.

• 

  IHC staining of rat testis using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 23418-1-AP (Osteocalcin Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of NIH/3T3 using 23418-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Osteocalcin antibody (23418-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of U2OS using 23418-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using Osteocalcin antibody (23418-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of NIH/3T3 using 23418-1-AP

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug Anti-Human Osteocalcin (23418-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IHC detected in: mouse testis tissue, human osteosarcoma tissue, human testis tissue, rat testis tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells, NIH/3T3 cells
• Positive FC (Intra) detected in: NIH/3T3 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 88 publications below
• IF: See 81 publications below

Product Information

23418-1-AP targets Osteocalcin/OCN in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Osteocalcin/OCN fusion protein Ag20065
• Full Name: bone gamma-carboxyglutamate (gla) protein
• Calculated Molecular Weight: 100 aa, 11 kDa
• GenBank Accession Number: BC113432
• Gene Symbol: Osteocalcin
• Gene ID (NCBI): 632
• RRID: AB_2879275
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Osteocalcin is a small, highly conserved molecule associated with mineralization of bone matrix. Osteocalcin is specifically expressed in osteoblasts and is the most abundant non-collagenous protein in bone. It regulates the dynamics of new bone formation and bone resorption by interaction with vitamin D, and by influencing the differentiation of osteoblasts. Osteocalcin is also involved in the posttranslational targeting of vitamin K-dependent gamma-carboxylation, which controls blood coagulation.

Publications

Species	Application	Title
mouse	IHC	Adv Mater; Supramolecular Hydrogel with Ultra-Rapid Cell-Mediated Network Adaptation for Enhancing Cellular Metabolic Energetics and Tissue Regeneration; Authors - Zhuo Li
mouse	IF	Bioact Mater; Ultrasound-generated bubbles enhance osteogenic differentiation of mesenchymal stromal cells in composite collagen hydrogels; Authors - Somnath Maji
human		Bioact Mater; 3D Printed Biomimetic Metamaterials with Graded Porosity and Tapering Topology for Improved Cell Seeding and Bone Regeneration; Authors - Lei Zhang
mouse	IHC,IF	ACS Nano; Nanotopography Sequentially Mediates Human Mesenchymal Stem Cell-Derived Small Extracellular Vesicles for Enhancing Osteogenesis.; Authors - Liang Ma
mouse	IF	Biomaterials; A nano-conductive osteogenic hydrogel to locally promote calcium influx for electro-inspired bone defect regeneration; Authors - Congcong Yu
mouse	IF	Sci Adv; Targeting actin-bundling protein L-plastin as an anabolic therapy for bone loss.; Authors - Xiaoqun Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

CHAO (Verified Customer) (03-31-2022)

Weak bands around the correct size

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Heart

FH

LUNFENG (Verified Customer) (01-27-2020)

GOOD

• Applications: Immunofluorescence,
• Cell Tissue Type: HEART

FH

Jie (Verified Customer) (01-23-2020)

Not working very well in my western blot

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse kidney tissue

FH

Canzhao (Verified Customer) (01-17-2020)

This antibody worked very well on western blot and immunofluorescence, very specific. I recommend this antibody!

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000 for WB, 1:100 for IF
• Cell Tissue Type: Mouse heart and cultured cardiomyocytes.

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