Osteocalcin Polyclonal antibody

Osteocalcin Polyclonal Antibody for IF, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 23418-1-AP

Synonyms

BGLAP, Osteocalcin, BGP, Bone Gla protein, OC, OCN, Osteocalcin, PMF1



Tested Applications

Positive IHC detected inhuman osteosarcoma tissue, human testis tissue, mouse kidney tissue, mouse testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 23418-1-AP is Osteocalcin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Osteocalcin fusion protein Ag20065
Full Name bone gamma-carboxyglutamate (gla) protein
Calculated molecular weight 100 aa, 11 kDa
GenBank accession numberBC113432
Gene symbol BGLAP
Gene ID (NCBI) 632
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for Osteocalcin antibody 23418-1-APDownload protocol
IF protocol for Osteocalcin antibody 23418-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

Colloids Surf B Biointerfaces

Bi-layered electrospun nanofibrous membrane with osteogenic and antibacterial properties for guided bone regeneration.

Authors - Meifei Lian
ratIF

FEBS J

Diminished membrane recruitment of Akt is instrumental in alcohol-associated osteopenia via the PTEN/Akt/GSK-3β/β-catenin axis.

Authors - Yi-Xuan Chen
ratWB

Arch Gynecol Obstet

Oviductus Ranae protein hydrolyzate prevents menopausal osteoporosis by regulating TGFβ/BMP2 signaling.

Authors - Xiaohua Li

Cell Death Differ

A novel long noncoding RNA PGC1β-OT1 regulates adipocyte and osteoblast differentiation through antagonizing miR-148a-3p.

Authors - Hairui Yuan
mouseIHC

J Cell Physiol

Reduced PDGF-AA in subchondral bone leads to articular cartilage degeneration after strenuous running.

Authors - Zilong Yao
mouseIHC

Nat Commun

TMCO1-mediated Ca2+ leak underlies osteoblast functions via CaMKII signaling.

Authors - Jianwei Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

LUNFENG (Verified Customer) (01-27-2020)

GOOD

  • Applications: Immunofluorescence,
  • Cell Tissue Type: HEART
FH

Jie (Verified Customer) (01-23-2020)

Not working very well in my western blot

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse kidney tissue
FH

Canzhao (Verified Customer) (01-17-2020)

This antibody worked very well on western blot and immunofluorescence, very specific. I recommend this antibody!

  • Applications: Western Blot,Immunofluorescence,
  • Primary Antibody Dilution: 1:1000 for WB, 1:100 for IF
  • Cell Tissue Type: Mouse heart and cultured cardiomyocytes.