Osteocalcin Polyclonal antibody
Osteocalcin Polyclonal Antibody for IF, IHC,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 23418-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive IHC detected in | human osteosarcoma tissue, human testis tissue, mouse kidney tissue, mouse testis tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 10 publications below |
| IHC | See 20 publications below |
| IF | See 23 publications below |
Product Information
The immunogen of 23418-1-AP is Osteocalcin Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Osteocalcin fusion protein Ag20065 |
| Full Name | bone gamma-carboxyglutamate (gla) protein |
| Calculated molecular weight | 100 aa, 11 kDa |
| GenBank accession number | BC113432 |
| Gene symbol | BGLAP |
| Gene ID (NCBI) | 632 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Osteocalcin antibody 23418-1-AP | Download protocol |
| IF protocol for Osteocalcin antibody 23418-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Colloids Surf B Biointerfaces Bi-layered electrospun nanofibrous membrane with osteogenic and antibacterial properties for guided bone regeneration. | ||
FEBS J Diminished membrane recruitment of Akt is instrumental in alcohol-associated osteopenia via the PTEN/Akt/GSK-3β/β-catenin axis. | ||
Arch Gynecol Obstet Oviductus Ranae protein hydrolyzate prevents menopausal osteoporosis by regulating TGFβ/BMP2 signaling. | ||
Cell Death Differ A novel long noncoding RNA PGC1β-OT1 regulates adipocyte and osteoblast differentiation through antagonizing miR-148a-3p. | ||
J Cell Physiol Reduced PDGF-AA in subchondral bone leads to articular cartilage degeneration after strenuous running. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH LUNFENG (Verified Customer) (01-27-2020) | GOOD
|
FH Jie (Verified Customer) (01-23-2020) | Not working very well in my western blot
|
FH Canzhao (Verified Customer) (01-17-2020) | This antibody worked very well on western blot and immunofluorescence, very specific. I recommend this antibody!
|
