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P21 Polyclonal antibody

P21 Polyclonal Antibody for IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 10240-1-AP

Synonyms

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human breast cancer tissue using P21 Polyclonal antibody (10240-1-AP)

    IHC staining of human breast cancer using 10240-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10240-1-AP (P21 antibody) at dilution of 1:50 (under 25x lens).

  • Immunofluorescence (IF) / fluorescent staining of myotome of a somite at E11.5 using P21 Polyclonal antibody (10240-1-AP)

    IF Staining of myotome of a somite at E11.5 using 10240-1-AP

    IF result of anti-P21(10240-1-AP) in the myotome of a somite at E11.5 by Dr. Zalc A. and Relaix F. (UPMC - UMRS 787).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using P21 Polyclonal antibody (10240-1-AP)

    IF Staining of SH-SY5Y using 10240-1-AP

    Immunofluorescent analysis of SH-SY5Y cells, using CDKN1A antibody 10240-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive IF detected inmyotome of a somite at E11.5, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10240-1-AP targets P21 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen P21 fusion protein Ag0308
Full Name cyclin-dependent kinase inhibitor 1A (p21, Cip1)
Calculated Molecular Weight 21 kDa
GenBank Accession NumberBC000312
Gene Symbol CDKN1A
Gene ID (NCBI) 1026
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDKN1A (p21, CIP1, WAF1), a cyclin-dependent kinase inhibitor, is necessary for proper control of the cell cycle and premature senescence. P21 is a cyclin-dependent kinase (CDK) inhibitor that suppresses proliferation by inhibiting CDK2 and CDK1 activity at the G1/S and G2/M transitions. P21 is a major mediator of p53 to induce cell cycle arrest in G1. p21 can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. P21 was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Overexpression of p21 decreases the expression levels of cell cycle progression genes and upregulates senescence-inducing genes.

Publications

SpeciesApplicationTitle
humanWB

PLoS One

FLASH knockdown sensitizes cells to Fas-mediated apoptosis via down-regulation of the anti-apoptotic proteins, MCL-1 and Cflip short.

Authors - Chen Song S
View Article