P4HA3 Polyclonal antibody

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  WB analysis using 23185-1-AP

  Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 23185-1-AP (P4HA3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 23185-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 23185-1-AP (P4HA3 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IF Staining of A549 using 23185-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using P4HA3 antibody (23185-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

P4HA3 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 23185-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

4 PH alpha 3, P4HA3

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Tested Applications

• Positive WB detected in: mouse kidney tissue, A549 cells
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 2 publications below

Product Information

23185-1-AP targets P4HA3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19716

  Product name: Recombinant human P4HA3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 28-256 aa of BC126170

  Sequence: GDTFSALTSVARALAPERRLLGLLRRYLRGEEARLRDLTRFYDKVLSLHEDSTTPVANPLLAFTLIKRLQSDWRNVVHSLEASENIRALKDGYEKVEQDLPAFEDLEGAARALMRLQDVYMLNVKGLARGVFQRVTGSAITDLYSPKRLFSLTGDDCFQVGKVAYDMGDYYHAIPWLEEAVSLFRGSYGEWKTEDEASLEDALDHLAFAYFRAGNVSCALSLSREFLLY

• Full Name: prolyl 4-hydroxylase, alpha polypeptide III
• Calculated Molecular Weight: 544 aa, 61 kDa
• Observed Molecular Weight: 30 kDa, 61 kDa
• GenBank Accession Number: BC126170
• Gene Symbol: P4HA3
• Gene ID (NCBI): 283208
• RRID: AB_2879228
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7Z4N8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Collagen prolyl 4-hydroxylases catalyze the hydroxylation of proline in x-pro-gly triplets in collagens and in proteins with collagen-like sequences. Collagen prolyl 4-hydroxylases are tetramers of 2 alpha subunits, such as P4HA3, and 2 beta subunits(P4HB). P4HA3 has 3 isoforms produced by alternative splicing with the MW of 61 kDa, 29 kDa and 67 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,rat	WB	J Mol Cell Cardiol; Ascorbate starvation alters endoplasmic reticulum-resident enzymes in cardiac fibroblasts, priming them for increased procollagen secretion.; Authors - Randy T Cowling
human	WB	Commun Biol; TGF-β-dependent reprogramming of amino acid metabolism induces epithelial-mesenchymal transition in non-small cell lung cancers.; Authors - Fumie Nakasuka ; KD Validated
mouse	IHC	J Dent Sci; Thrombospondin-1 and prolyl 4-hydroxylase subunit alpha 3 as potential biomarkers of salivary gland fibrosis; Authors - Zijian Zhang
human	WB	bioRxiv; Identifying and targeting key driver genes for collagen production within the 11q13/14 breast cancer amplicon; Authors - Daniela Araiza-Olivera ; KD Validated
human	IHC	Heliyon; Collagen synthase P4HA3 as a novel biomarker for colorectal cancer correlates with prognosis and immune infiltration; Authors - Xiaohuan Guo
mouse	WB	Commun Biol; Protein disulfide isomerase is essential for osteoblast differentiation in mice; Authors - Yue Lu