P4HA3 Polyclonal antibody

P4HA3 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 23185-1-AP

Synonyms

4 PH alpha 3, P4HA3

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Validation Data Gallery

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  WB analysis of mouse kidney using 23185-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 23185-1-AP (P4HA3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 23185-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 23185-1-AP (P4HA3 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IF Staining of A549 using 23185-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 23185-1-AP (P4HA3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, A549 cells
• Positive IF detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below

Product Information

The immunogen of 23185-1-AP is P4HA3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: P4HA3 fusion protein Ag19716
• Full Name: prolyl 4-hydroxylase, alpha polypeptide III
• Calculated molecular weight: 544 aa, 61 kDa
• Observed molecular weight: 30 kDa, 61 kDa
• GenBank accession number: BC126170
• Gene symbol: P4HA3
• Gene ID (NCBI): 283208
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Collagen prolyl 4-hydroxylases catalyze the hydroxylation of proline in x-pro-gly triplets in collagens and in proteins with collagen-like sequences. Collagen prolyl 4-hydroxylases are tetramers of 2 alpha subunits, such as P4HA3, and 2 beta subunits(P4HB). P4HA3 has 3 isoforms produced by alternative splicing with the MW of 61 kDa, 29 kDa and 67 kDa.

Publications

Species	Application	Title
mouse,rat	WB	J Mol Cell Cardiol; Ascorbate starvation alters endoplasmic reticulum-resident enzymes in cardiac fibroblasts, priming them for increased procollagen secretion.; Authors - Randy T Cowling
human	WB	Commun Biol; TGF-β-dependent reprogramming of amino acid metabolism induces epithelial-mesenchymal transition in non-small cell lung cancers.; Authors - Fumie Nakasuka ; KD Validated
human	WB	Mol Cell Biochem; Transcriptome sequencing identifies prognostic genes involved in gastric adenocarcinoma; Authors - Mingyue Li ; KD Validated