P4HA3 Polyclonal antibody

P4HA3 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 23185-1-AP

Synonyms

4 PH alpha 3, P4HA3



Tested Applications

Positive WB detected inmouse kidney tissue, A549 cells
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 23185-1-AP is P4HA3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen P4HA3 fusion protein Ag19716
Full Name prolyl 4-hydroxylase, alpha polypeptide III
Calculated molecular weight 544 aa, 61 kDa
Observed molecular weight 30 kDa, 61 kDa
GenBank accession numberBC126170
Gene symbol P4HA3
Gene ID (NCBI) 283208
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Collagen prolyl 4-hydroxylases catalyze the hydroxylation of proline in x-pro-gly triplets in collagens and in proteins with collagen-like sequences. Collagen prolyl 4-hydroxylases are tetramers of 2 alpha subunits, such as P4HA3, and 2 beta subunits(P4HB). P4HA3 has 3 isoforms produced by alternative splicing with the MW of 61 kDa, 29 kDa and 67 kDa.

Protocols

Product Specific Protocols
WB protocol for P4HA3 antibody 23185-1-APDownload protocol
IF protocol for P4HA3 antibody 23185-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,ratWB

J Mol Cell Cardiol

Ascorbate starvation alters endoplasmic reticulum-resident enzymes in cardiac fibroblasts, priming them for increased procollagen secretion.

Authors - Randy T Cowling