P4HA3 Polyclonal antibody
P4HA3 Polyclonal Antibody for IF, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IF, ELISA
Conjugate
Unconjugated
Cat no : 23185-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse kidney tissue, A549 cells |
Positive IF detected in | A549 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 3 publications below |
Product Information
The immunogen of 23185-1-AP is P4HA3 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | P4HA3 fusion protein Ag19716 |
Full Name | prolyl 4-hydroxylase, alpha polypeptide III |
Calculated molecular weight | 544 aa, 61 kDa |
Observed molecular weight | 30 kDa, 61 kDa |
GenBank accession number | BC126170 |
Gene symbol | P4HA3 |
Gene ID (NCBI) | 283208 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Collagen prolyl 4-hydroxylases catalyze the hydroxylation of proline in x-pro-gly triplets in collagens and in proteins with collagen-like sequences. Collagen prolyl 4-hydroxylases are tetramers of 2 alpha subunits, such as P4HA3, and 2 beta subunits(P4HB). P4HA3 has 3 isoforms produced by alternative splicing with the MW of 61 kDa, 29 kDa and 67 kDa.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for P4HA3 antibody 23185-1-AP | Download protocol |
IF protocol for P4HA3 antibody 23185-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Mol Cell Cardiol Ascorbate starvation alters endoplasmic reticulum-resident enzymes in cardiac fibroblasts, priming them for increased procollagen secretion. | ||
Commun Biol TGF-β-dependent reprogramming of amino acid metabolism induces epithelial-mesenchymal transition in non-small cell lung cancers.
| ||
Mol Cell Biochem Transcriptome sequencing identifies prognostic genes involved in gastric adenocarcinoma
|