PABPN1 Monoclonal antibody

• Featured Product
• KD/KO Validated

PABPN1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

5

CloneNo.

3C12E12

Cat no : 66807-1-Ig

Synonyms

OPMD, PAB2, PABII, PABP 2, PABP2, PABPN1, Poly(A) binding protein 2, Poly(A) binding protein II

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Validation Data Gallery

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  WB analysis using 66807-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66807-1-Ig (PABPN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66807-1-Ig

  WB result of PABPN1 antibody (66807-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PABPN1 transfected HeLa cells.

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  WB analysis using 66807-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66807-1-Ig (PABPN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 66807-1-Ig

  IP result of anti-PABPN1 (IP:66807-1-Ig, 5ug; Detection:66807-1-Ig 1:600) with Jurkat cells lysate 1680 ug.

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  IHC staining of human colon using 66807-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66807-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66807-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 66807-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 66807-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 66807-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 66807-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 66807-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 66807-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PABPN1 antibody (66807-1-Ig, Clone: 3C12E12 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, HEK-293 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, K-562 cells, PC-12 cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human colon tissue, human colon cancer tissue, rat heart tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Product Information

66807-1-Ig targets PABPN1 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PABPN1 fusion protein Ag27470
• Full Name: poly(A) binding protein, nuclear 1
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 50-56 kDa
• GenBank Accession Number: BC010939
• Gene Symbol: PABPN1
• Gene ID (NCBI): 8106
• RRID: AB_2882150
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Poly(A) Binding Protein Nuclear 1(PABPN1) is one of the isoforms of poly(A) binding protein (PABP) that locating to the nucleus in eukaryotic cells. And PABPN1 plays important role in mRNA stability by regulating of poly-A tail length, mRNA decay and proximal polyadenylation site (PAS) utilization at the 3'-UTR (PMID:23300856). Another report showed that PABPN1 shuttles between the nucleus and the cytoplasm to export poly(A) RNA from the nucleus (PMID:23601051). It is reported that PABPN1 is ubiquitously expressed in almost all tissues, however, mutations in PABPN1 causes Oculopharyngeal Muscular Dystrophy (OPMD) which affected in skeletal muscles specifically. Because of a mild reduction in PABPN1 levels is sufficient to induce muscle wasting (PMID:27152426). Acetylation, Methylation, Phosphoprotein are common post-translational modifications of PABPN1 protein. And 50-55 kDa bands have been reported (PMID: 29939290; 28361972).

Publications

Species	Application	Title
human	WB	Cell Rep; Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome.; Authors - J J David Ho
mouse	WB	J Biol Chem; Extracellular 5'-methylthioadenosine inhibits intracellular symmetric dimethylarginine protein methylation of FUSE-element binding proteins; Authors - Baiqing Tang
human	WB,IHC,RIP	Cell Biosci; PABPN1 regulates mRNA alternative polyadenylation to inhibit bladder cancer progression; Authors - Liang Chen ; KD Validated
human	WB	Nat Cell Biol; An mRNA processing pathway suppresses metastasis by governing translational control from the nucleus; Authors - Albertas Navickas
human,mouse	WB,CoIP	Mol Cell; The MYCN oncoprotein is an RNA-binding accessory factor of the nuclear exosome targeting complex; Authors - Dimitrios Papadopoulos