PAK2 Polyclonal antibody

PAK2 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Reviews (1)

Cat no : 19979-1-AP

Synonyms

C t PAK2, Gamma PAK, p21 activated kinase 2, p27 PAK 2p34, p34, p58, PAK 2, PAK2, PAK65, PAKgamma, S6/H4 kinase

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Validation Data Gallery

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  WB analysis using 19979-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19979-1-AP (PAK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 19979-1-AP

  Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 19979-1-AP (PAK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 19979-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19979-1-AP (PAK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 19979-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PAK2 antibody (19979-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, human skeletal muscle tissue, Jurkat cells, HeLa cells, PC-3 cells, Raji cells
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19979-1-AP targets PAK2 in WB, IF,ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: p21 protein (Cdc42/Rac)-activated kinase 2
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: NM_002577
• Gene Symbol: PAK2
• Gene ID (NCBI): 5062
• RRID: AB_10644144
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAK2, also named as PAK65, PAKgamma, p58, PAK-2p27, PAK-2p24 and C-t-PAK2, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. PAK2 has several isoforms with the MW of 54-62 kDa and 41 kDa. The 62 kDa PAK2 is cleaved into a 34 kDa C terminal fragment and a 28 kDa N terminal fragment with a time course that parallels apoptotic death in Jurkat cells. (PMID:10200518). Caspase-activated PAK-2p34 is involved in cell death response, probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated form. The antibody has no cross reaction to PAK1 and PAK3.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Frédéric (Verified Customer) (02-18-2022)

Je suis satisfait du résultat car j’ai pu détecter par Western-blot une seule bande de forte intensité d’environ 69 kDa sur un lysat de plaquette humaine (voir figure ci-jointe).

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: lysat de plaquette humaine

PAK2 Antibody Western Blot validation (1/1000 dilution) in lysat de plaquette humaine (Cat no:19979-1-AP)