PAX3 Polyclonal antibody

PAX3 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 21386-1-AP

Synonyms

CDHS, HUP2, paired box 3, Paired box protein Pax 3, PAX3, WS1



Tested Applications

Positive WB detected inmouse skin tissue, HEK-293 cells
Positive IP detected inmouse skin tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

The immunogen of 21386-1-AP is PAX3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAX3 fusion protein Ag16021
Full Name paired box 3
Calculated Molecular Weight 484 aa, 53 kDa
Observed Molecular Weight 56-60 kDa
GenBank Accession NumberBC101301
Gene Symbol PAX3
Gene ID (NCBI) 5077
RRIDAB_10732817
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAX3, a transcription factor and multifunctional regulatory protein, is normally expressed during embryonic development. In the nervous system, PAX3 is involved in neural tube closure, neural crest development, and peripheral neuron differentiation. In the present study, PAX3 was reported as a novel regulator of GFAP transcription, and the overexpression and suppression of PAX3 could inhibit and promote NSC differentiation, respectively. In muscle development, PAX3 ensures the survival of myogenic progenitor cells with Pax3-expressing progenitors giving rise to both skeletal and smooth muscle cells. PAX3 also has a well-established role in the development of melanocytes during embryogenesis, and has recently been characterized as a molecular switch in the mature melanocyte. Mutations in PAX3 can cause Waardenburg syndrome.

Protocols

Product Specific Protocols
WB protocol for PAX3 antibody 21386-1-APDownload protocol
IP protocol for PAX3 antibody 21386-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
hamsterIHC

Birth Defects Res

Cigarette smoke induced neural tube defects by down-regulating noggin expression.

Authors - Chuanhui Yin