PAX6 Monoclonal antibody

PAX6 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

2C5A1

Reviews (1)

Cat no : 67529-1-Ig

Synonyms

Pax-6, PAX 6, Paired box protein Pax-6, Paired box protein Pax 6, paired box 6

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Validation Data Gallery

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  WB analysis using 67529-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat cerebellum using 67529-1-Ig

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67529-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67529-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of JAR using 67529-1-Ig

  JAR cells were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of hTERT-RPE1 using 67529-1-Ig

  hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67529-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 67529-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse eye using 67529-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse eye using 67529-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 67529-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 67529-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67529-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67529-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A-673 cells, HeLa cells, hTERT-RPE1 cells, JAR cells, K-562 cells, Neuro-2a cells, rat cerebellum tissue, SW480 cells, HT-1376 cells, MG-63 cells, rat retina tissue, mouse retina tissue, rabbit retina tissue
• Positive IHC detected in: mouse eye tissue, mouse brain tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 4 publications below

Product Information

67529-1-Ig targets PAX6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PAX6 fusion protein Ag29977
• Full Name: paired box 6
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC011953
• Gene Symbol: PAX6
• Gene ID (NCBI): 5080
• RRID: AB_2882748
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAX6, a paired domain and homeodomain-containing transcription factor. Interaction with TRIM11 leads to ubiquitination of PAX6 and its proteasomal degradation. PAX6 is one of the earliest genes expressed in the eye field and considered a master control gene for retinal and eye development. PAX6 also regulates the development of the olfactory, central nervous systems, pituitary, and pancreas. PAX6 mutations can cause complex ocular disorders such as aniridia and Peter's anomaly.

Publications

Species	Application	Title
human	IF	Anal Bioanal Chem; Simultaneous and quantitative monitoring transcription factors in human embryonic stem cell differentiation using mass spectrometry-based targeted proteomics.; Authors - Mengying Xu
human	IF	Stem Cell Res; Generation of KCNH2 heterozygous knockout induced pluripotent stem cell (iPSC) line (Long and Short QT Syndrome); Authors - Baiqiang Wang
rat	IF	Neural Regen Res; Müller cells are activated in response to retinal outer nuclear layer degeneration in rats subjected to simulated weightlessness conditions; Authors - Yuxue Mu
human	IF	Stem Cell Res; Generation of an induced pluripotent stem cell line (HMSCATi004-A) from an early onset Parkinson's disease patient with PRKN gene mutation; Authors - Jingke Cheng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Raquel (Verified Customer) (07-12-2024)

Immunofluorescence analysis in cryostat sections of 4% PFA fixed human brain organoid derived from iPSCs with 67529-1-Ig PAX6 antibody at dilution of 1:100 (under 20x lens). Antigen retrieval with Citrate buffer 10mM pH6. Green:Pax6 ; Blue: DAPI. Although most of the 67529-1-Ig immunostained cells colocalize with Pax6 positive cells, few of them seem to not colocalize.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human brain organoid

PAX6 Antibody Immunofluorescence validation (1:100 dilution) in Human brain organoid (Cat no:67529-1-Ig)