Tested Applications
| Positive WB detected in | A-673 cells, HeLa cells, hTERT-RPE1 cells, JAR cells, K-562 cells, Neuro-2a cells, rat cerebellum tissue, SW480 cells, HT-1376 cells, MG-63 cells, rat retina tissue, mouse retina tissue, rabbit retina tissue |
| Positive IHC detected in | mouse eye tissue, mouse brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 5 publications below |
Product Information
67529-1-Ig targets PAX6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | PAX6 fusion protein Ag29977 Predict reactive species |
| Full Name | paired box 6 |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 47 kDa |
| GenBank Accession Number | BC011953 |
| Gene Symbol | PAX6 |
| Gene ID (NCBI) | 5080 |
| RRID | AB_2882748 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P26367 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PAX6, a paired domain and homeodomain-containing transcription factor. Interaction with TRIM11 leads to ubiquitination of PAX6 and its proteasomal degradation. PAX6 is one of the earliest genes expressed in the eye field and considered a master control gene for retinal and eye development. PAX6 also regulates the development of the olfactory, central nervous systems, pituitary, and pancreas. PAX6 mutations can cause complex ocular disorders such as aniridia and Peter's anomaly.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PAX6 antibody 67529-1-Ig | Download protocol |
| IHC protocol for PAX6 antibody 67529-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Anal Bioanal Chem Simultaneous and quantitative monitoring transcription factors in human embryonic stem cell differentiation using mass spectrometry-based targeted proteomics. | ||
Stem Cell Res Generation of KCNH2 heterozygous knockout induced pluripotent stem cell (iPSC) line (Long and Short QT Syndrome) | ||
Neural Regen Res Müller cells are activated in response to retinal outer nuclear layer degeneration in rats subjected to simulated weightlessness conditions | ||
Stem Cell Res Generation of an induced pluripotent stem cell line (HMSCATi004-A) from an early onset Parkinson's disease patient with PRKN gene mutation | ||
Stem Cell Res Generation of heterozygous KCNA2 knockout induced pluripotent stem cell line |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Raquel (Verified Customer) (07-12-2024) | Immunofluorescence analysis in cryostat sections of 4% PFA fixed human brain organoid derived from iPSCs with 67529-1-Ig PAX6 antibody at dilution of 1:100 (under 20x lens). Antigen retrieval with Citrate buffer 10mM pH6. Green:Pax6 ; Blue: DAPI. Although most of the 67529-1-Ig immunostained cells colocalize with Pax6 positive cells, few of them seem to not colocalize.
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