PAX6 Monoclonal antibody
PAX6 Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
2C5A1
Cat no : 67529-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | rat retina tissue, JAR cells, hTERT-RPE1 cells, HeLa cells, K-562 cells, Neuro-2a cells |
Positive IHC detected in | mouse eye tissue, mouse brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Published Applications
IF | See 1 publications below |
Product Information
67529-1-Ig targets PAX6 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Reactivity | Human, mouse, rat |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | PAX6 fusion protein Ag29977 |
Full Name | paired box 6 |
Calculated molecular weight | 47 kDa |
Observed molecular weight | 47 kDa |
GenBank accession number | BC011953 |
Gene symbol | PAX6 |
Gene ID (NCBI) | 5080 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PAX6, a paired domain and homeodomain-containing transcription factor. Interaction with TRIM11 leads to ubiquitination of PAX6 and its proteasomal degradation. PAX6 is one of the earliest genes expressed in the eye field and considered a master control gene for retinal and eye development. PAX6 also regulates the development of the olfactory, central nervous systems, pituitary, and pancreas. PAX6 mutations can cause complex ocular disorders such as aniridia and Peter's anomaly.
Protocols
Product Specific Protocols | |
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WB protocol for PAX6 antibody 67529-1-Ig | Download protocol |
IHC protocol for PAX6 antibody 67529-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Anal Bioanal Chem Simultaneous and quantitative monitoring transcription factors in human embryonic stem cell differentiation using mass spectrometry-based targeted proteomics. |