|Positive WB detected in||SKOV-3 cells, HEK-293 cells, NIH/3T3 cells, Raji cells|
|Positive IHC detected in||human ovary tumor tissue, human thyroid cancer tissue, human renal cell carcinoma tissue, mouse kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||SKOV-3 cells, mouse kidney tissue|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:5000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10336-1-AP targets PAX8 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||bovine, human, mouse, rat, swine, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PAX8 fusion protein Ag0306|
|Full Name||paired box 8|
|Calculated molecular weight||48 kDa|
|Observed molecular weight||48 kDa, 58 kDa|
|GenBank accession number||BC001060|
|Gene ID (NCBI)||7849|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas.
This antibody can detect all 5 isoforms of PAX8 (31 kDa, 34 kDa, 41 kDa, 43 kDa and 48 kDa). A 58-60 kDa form has also been reported (PMID:15650356, 1049952). Proteintech's 10336-1-AP antibody has cross-reactivities with the other PAX family members.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Eunyoung (Verified Customer) (02-28-2020)
This antibody works well on the mouse ovarian cancer tissue
shuang (Verified Customer) (02-18-2020)
This Pax8 antibody works great for both IHC and IF staining on mouse Fallopian tube/uterus organoids and tumors, Highly recommend!
Adam (Verified Customer) (05-30-2019)
Antibody works great for our applications (IHC on FFPE tissues). Only comment is that it cane sometimes have some background staining, particularly in some mouse tissues such as liver.
Huai-Chin (Verified Customer) (04-15-2019)
So far only try the antibody for IHC on mouse tissue. Works pretty good. Would like to try it on human tissue/cell lines.
Paulo (Verified Customer) (03-03-2019)
Used to perform ChIP-seq in ccRCC cell lines. 17.5ug of antibody used in 100million cells. IP was validated by WB. Sequencing detected PAX8 binding genome wide. Motif analysis identified the consensus PAX8 binding motif in approximately 90% of detected peaks. Would be great if Proteintech would produce a more concentrated version of this antibody for ChIP-seq.