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PAX8 Polyclonal antibody

PAX8 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10336-1-AP

Synonyms

paired box 8, Paired box protein Pax 8, PAX8



Tested Applications

Positive WB detected inSKOV-3 cells, HEK-293 cells, NIH/3T3 cells, Raji cells
Positive IHC detected inhuman ovary tumor tissue, human thyroid cancer tissue, human renal cell carcinoma tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSKOV-3 cells, mouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:5000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10336-1-AP targets PAX8 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity bovine, human, mouse, rat, swine, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAX8 fusion protein Ag0306
Full Name paired box 8
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa, 58 kDa
GenBank accession numberBC001060
Gene symbol PAX8
Gene ID (NCBI) 7849
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas.

This antibody can detect all 5 isoforms of PAX8 (31 kDa, 34 kDa, 41 kDa, 43 kDa and 48 kDa). A 58-60 kDa form has also been reported (PMID:15650356, 1049952). Proteintech's 10336-1-AP antibody has cross-reactivities with the other PAX family members.

Protocols

Product Specific Protocols
WB protocol for PAX8 antibody 10336-1-APDownload protocol
IHC protocol for PAX8 antibody 10336-1-APDownload protocol
IF protocol for PAX8 antibody 10336-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Virchows Arch

Histological and molecular characterization of TFEB-rearranged renal cell carcinomas.

Authors - Nicolas Wyvekens
humanIHC

Commun Biol

A simple high-throughput approach identifies actionable drug sensitivities in patient-derived tumor organoids.

Authors - Nhan Phan
humanIHC

Hum Pathol

MDM2 amplification and immunohistochemical expression in sarcomatoid renal cell carcinoma.

Authors - David Suster
humanIHC

Reprod Sci

PAX8: A Highly Sensitive Marker for the Glands in Extragenital Endometriosis.

Authors - Tomoko Arakawa
humanIF

Nat Mater

Fine tuning the extracellular environment accelerates the derivation of kidney organoids from human pluripotent stem cells.

Authors - Elena Garreta
humanIHC

Reprod Sci

IFITM1 is a Novel, Highly Sensitive Marker for Endometriotic Stromal Cells in Ovarian and Extragenital Endometriosis.

Authors - Hui Sun

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Eunyoung (Verified Customer) (02-28-2020)

This antibody works well on the mouse ovarian cancer tissue

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Ovary /fallopian tissue
PAX8 Antibody Immunohistochemistry, validation (1:1000 dilution) in Ovary /fallopian tissue (Cat no:10336-1-AP)
FH

shuang (Verified Customer) (02-18-2020)

This Pax8 antibody works great for both IHC and IF staining on mouse Fallopian tube/uterus organoids and tumors, Highly recommend!

  • Applications: Immunohistochemistry,Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: mouse Fallopian tube/ Fallopian tube organoids
FH

Adam (Verified Customer) (05-30-2019)

Antibody works great for our applications (IHC on FFPE tissues). Only comment is that it cane sometimes have some background staining, particularly in some mouse tissues such as liver.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:1000
FH

Huai-Chin (Verified Customer) (04-15-2019)

So far only try the antibody for IHC on mouse tissue. Works pretty good. Would like to try it on human tissue/cell lines.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse Ovary/Oviduct
FH

Paulo (Verified Customer) (03-03-2019)

Used to perform ChIP-seq in ccRCC cell lines. 17.5ug of antibody used in 100million cells. IP was validated by WB. Sequencing detected PAX8 binding genome wide. Motif analysis identified the consensus PAX8 binding motif in approximately 90% of detected peaks. Would be great if Proteintech would produce a more concentrated version of this antibody for ChIP-seq.

  • Applications: ChIP,