CoraLite®488-conjugated PAX8 Polyclonal antibody

PAX8 Polyclonal Antibody for FC, IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, FC

Conjugate

CoraLite®488 Fluorescent Dye

Cat no : CL488-10336

Synonyms

paired box 8, Paired box protein Pax 8, PAX8



Tested Applications

Positive IF detected inSKOV-3 cells
Positive FC detected inSKOV-3 cells
For other applications, we recommend the unconjugated version of this antibody, 10336-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-10336 targets PAX8 in IF, FC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAX8 fusion protein Ag0306
Full Name paired box 8
Calculated molecular weight 48 kDa
GenBank accession numberBC001060
Gene symbol PAX8
Gene ID (NCBI) 7849
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas.

This antibody can detect all 5 isoforms of PAX8 (31 kDa, 34 kDa, 41 kDa, 43 kDa and 48 kDa). A 58-60 kDa form has also been reported (PMID:15650356, 1049952). Proteintech's 10336-1-AP antibody has cross-reactivities with the other PAX family members.

Protocols

Product Specific Protocols
IF protocol for CL488 PAX8 antibody CL488-10336Download protocol
FC protocol for CL488 PAX8 antibody CL488-10336Download protocol
Standard Protocols
Click here to view our Standard Protocols