PBXIP1 Polyclonal antibody

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  WB analysis of BxPC-3 using 12102-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12102-1-AP (PBXIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12102-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12102-1-AP (PBXIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12102-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12102-1-AP (PBXIP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12102-1-AP

  IP result of anti-PBXIP1 (IP:12102-1-AP, 3ug; Detection:12102-1-AP 1:800) with HeLa cells lysate 3000ug.

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  IHC staining of human lung cancer using 12102-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12102-1-AP (PBXIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 12102-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12102-1-AP (PBXIP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 12102-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PBXIP1 antibody (12102-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 12102-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PBXIP1 antibody (12102-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

PBXIP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12102-1-AP

Publications(14)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, chIP, ELISA

Synonyms

Pre-B-cell leukemia transcription factor-interacting protein 1, HPIP, Hematopoietic PBX-interacting protein

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Tested Applications

• Positive WB detected in: BxPC-3 cells, A549 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 11 publications below
• IHC: See 5 publications below
• IF: See 3 publications below
• ChIP: See 1 publications below

Product Information

12102-1-AP targets PBXIP1 in WB, IHC, IF/ICC, IP, chIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2746

  Product name: Recombinant human PBXIP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-300 aa of BC016852

  Sequence: MASCPDSDNSWVLAGSESLPVETLGPASRMDPESERALQAPHSPSKTDGKELAGTMDGEGTLFQTESPQSGSILTEETEVKGTLEGDVCGVEPPGPGDTVVQGDLQETTVVTGLGPDTQDLEGQSPPQSLPSTPKAAWIREEGRCSSSDDDTDVDMEGLRRRRGREAGPPQPMVPLAVENQAGGEGAGGELGISLNMCLLGALVLLGLGVLLFSGGLSESETGPMEEVERQVLPDPEVLEAVGDRQDGLREQLQAPVPPDSVPSLQNMGLLLDKLAKENQDIRLLQAQLQAQKEELQSLM

• Full Name: pre-B-cell leukemia homeobox interacting protein 1
• Calculated Molecular Weight: 731 aa, 81 kDa
• Observed Molecular Weight: 95-100 kDa
• GenBank Accession Number: BC016852
• Gene Symbol: PBXIP1
• Gene ID (NCBI): 57326
• RRID: AB_2160484
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96AQ6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PBXIP1 (pre-B-cell leukemia transcription factor-interacting protein 1), also known as HPIP (hematopoietic PBX-interacting protein), is a regulator of pre-B-cell leukemia transcription factors (BPXs) function. PBXIP1 is a nucleo-cytoplasmic shuttling protein, mainly localized in the cytosol and in small amounts in the nucleus (PMID: 10825160). It interacts with PBX1 as well as PBX2 and PBX3. PBXIP1 inhibits the ability of PBX-HOX heterodimers to bind to target sequences and strongly inhibits the transactivation activity of E2A-PBX. PBXIP1 is also reported to be a microtubule-binding protein, which regulates estrogen receptor functions and plays a role in cancer development and progression (PMID: 17043237; 18302941; 23321675). The predicted PBXIP1 protein has a calculated molecular mass of 81 kDa. This polyclonal antibody raised against 1-300aa of human PBXIP1 recognizes endogenous PBXIP1 with an apparent molecular weight of 95-100 kDa. The slow migration of PBXIP1 possibly results from either post-translational modifications or intrinsic SDS-resistant folding of the protein (PMID: 10825160; 17043237).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Clin Invest; Hepatitis B virus X protein represses miRNA-148a to enhance tumorigenesis.; Authors - Xu Xiaojie X ; KD Validated
human	WB,IF	Cell Death Differ; MDM2 restrains estrogen-mediated AKT activation by promoting TBK1-dependent HPIP degradation.; Authors - K Shostak
	WB	Hum Genet; Human organoids for rapid validation of gene variants linked to cochlear malformations; Authors - Mohammad Faraz Zafeer
human	WB,IHC,IF	Oncogenesis; The interplay between HPIP and casein kinase 1α promotes renal cell carcinoma growth and metastasis via activation of mTOR pathway.; Authors - H Mai ; KD Validated
human	WB,IHC	Cancer Sci; Hematopoietic pre-B cell leukemia transcription factor interacting protein is overexpressed in gastric cancer and promotes gastric cancer cell proliferation, migration, and invasion.; Authors - Yingying Feng ; KD Validated
human, mouse	WB, IHC	Sci Rep; HPIP is upregulated in colorectal cancer and regulates colorectal cancer cell proliferation, apoptosis and invasion.; Authors - Yingying Feng