Tested Applications
| Positive WB detected in | BxPC-3 cells, A549 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12102-1-AP targets PBXIP1 in WB, IHC, IF/ICC, IP, chIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2746 Product name: Recombinant human PBXIP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC016852 Sequence: MASCPDSDNSWVLAGSESLPVETLGPASRMDPESERALQAPHSPSKTDGKELAGTMDGEGTLFQTESPQSGSILTEETEVKGTLEGDVCGVEPPGPGDTVVQGDLQETTVVTGLGPDTQDLEGQSPPQSLPSTPKAAWIREEGRCSSSDDDTDVDMEGLRRRRGREAGPPQPMVPLAVENQAGGEGAGGELGISLNMCLLGALVLLGLGVLLFSGGLSESETGPMEEVERQVLPDPEVLEAVGDRQDGLREQLQAPVPPDSVPSLQNMGLLLDKLAKENQDIRLLQAQLQAQKEELQSLM Predict reactive species |
| Full Name | pre-B-cell leukemia homeobox interacting protein 1 |
| Calculated Molecular Weight | 731 aa, 81 kDa |
| Observed Molecular Weight | 95-100 kDa |
| GenBank Accession Number | BC016852 |
| Gene Symbol | PBXIP1 |
| Gene ID (NCBI) | 57326 |
| RRID | AB_2160484 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96AQ6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PBXIP1 (pre-B-cell leukemia transcription factor-interacting protein 1), also known as HPIP (hematopoietic PBX-interacting protein), is a regulator of pre-B-cell leukemia transcription factors (BPXs) function. PBXIP1 is a nucleo-cytoplasmic shuttling protein, mainly localized in the cytosol and in small amounts in the nucleus (PMID: 10825160). It interacts with PBX1 as well as PBX2 and PBX3. PBXIP1 inhibits the ability of PBX-HOX heterodimers to bind to target sequences and strongly inhibits the transactivation activity of E2A-PBX. PBXIP1 is also reported to be a microtubule-binding protein, which regulates estrogen receptor functions and plays a role in cancer development and progression (PMID: 17043237; 18302941; 23321675). The predicted PBXIP1 protein has a calculated molecular mass of 81 kDa. This polyclonal antibody raised against 1-300aa of human PBXIP1 recognizes endogenous PBXIP1 with an apparent molecular weight of 95-100 kDa. The slow migration of PBXIP1 possibly results from either post-translational modifications or intrinsic SDS-resistant folding of the protein (PMID: 10825160; 17043237).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PBXIP1 antibody 12102-1-AP | Download protocol |
| IHC protocol for PBXIP1 antibody 12102-1-AP | Download protocol |
| IP protocol for PBXIP1 antibody 12102-1-AP | Download protocol |
| WB protocol for PBXIP1 antibody 12102-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
12102-1-AP is an anti-HPIP antibody with 15 total citations spanning journals including Nature Communications, JCI, Science Advances, Cell Death & Differentiation, Oncogenesis, and Cancer Science. Research fields cover oncology (liver, gastric, colorectal, renal, breast, and thyroid cancers), osteoarthritis, EGFR-RAS-ERK and mTOR signaling pathways, ubiquitin-proteasome system in neurodegeneration, and organoid models for cochlear malformations.
| Species | Application | Title |
|---|---|---|
Nat Commun Hematopoietic PBX-interacting protein mediates cartilage degeneration during the pathogenesis of osteoarthritis.
| ||
J Clin Invest Hepatitis B virus X protein represses miRNA-148a to enhance tumorigenesis.
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Sci Adv HPIP is an essential scaffolding protein running through the EGFR-RAS-ERK pathway and drives tumorigenesis | ||
Cell Death Differ MDM2 restrains estrogen-mediated AKT activation by promoting TBK1-dependent HPIP degradation. | ||
Cell Death Discov A novel chemical inhibitor suppresses breast cancer cell growth and metastasis through inhibiting HPIP oncoprotein. | ||
Oncogenesis The interplay between HPIP and casein kinase 1α promotes renal cell carcinoma growth and metastasis via activation of mTOR pathway.
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Reviews
What customers say
One user rated this antibody 5 stars for immunofluorescence on mouse neural stem cells at a 1:500 dilution, reporting clear and specific staining with excellent results.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
joey (Verified Customer) (04-27-2026) | It works clearly and gives a specific staining on mouse neural stem cells, thank you !
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