PCDH10 Polyclonal antibody

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  WB analysis of PC-3 using 21859-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 21859-1-AP (PCDH10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 21859-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21859-1-AP (PCDH10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 21859-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21859-1-AP (PCDH10 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 21859-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21859-1-AP (PCDH10 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 21859-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 21859-1-AP (PCDH10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

PCDH10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 21859-1-AP

Publications(5)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

KIAA1400, Protocadherin-10

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Tested Applications

• Positive WB detected in: SK-N-SH cells, PC-3 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Product Information

21859-1-AP targets PCDH10 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16494

  Product name: Recombinant human PCDH10 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 19-368 aa of BC111560

  Sequence: QLHYTVQEEQEHGTFVGNIAEDLGLDITKLSARGFQTVPNSRTPYLDLNLETGVLYVNEKIDREQICKQSPSCVLHLEVFLENPLELFQVEIEVLDINDNPPSFPEPDLTVEISESATPGTRFPLESAFDPDVGTNSLRDYEITPNSYFSLDVQTQGDGNRFAELVLEKPLDREQQAVHRYVLTAVDGGGGGGVGEGGGGGGGAGLPPQQQRTGTALLTIRVLDSNDNVPAFDQPVYTVSLPENSPPGTLVIQLNATDPDEGQNGEVVYSFSSHISPRARELFGLSPRTGRLEVSGELDYEESPVYQVYVQAKDLGPNAVPAHCKVLVRVLDANDNAPEISFSTVKEAVS

• Full Name: protocadherin 10
• Calculated Molecular Weight: 1040 aa, 113 kDa
• Observed Molecular Weight: 113-130 kDa
• GenBank Accession Number: BC111560
• Gene Symbol: PCDH10
• Gene ID (NCBI): 57575
• RRID: AB_2878929
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9P2E7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Theranostics; A Compound AC1Q3QWB Selectively Disrupts HOTAIR-Mediated Recruitment of PRC2 and Enhances Cancer Therapy of DZNep.; Authors - Yansheng Li
human	WB	Clin Transl Med; Hsa_circ_0001666 suppresses the progression of colorectal cancer through the miR-576-5p/PCDH10 axis.; Authors - Jiahui Zhou
human	WB	Int J Biol Sci; FOXM1-induced miR-552 expression contributes to pancreatic cancer progression by targeting multiple tumor suppressor genes.; Authors - Xiao Wang ; KD Validated
human	IF	Nature; Shifts in receptors during submergence of an encephalitic arbovirus; Authors - Wanyu Li
		Cell; Structural basis for plasticity in receptor engagement by an encephalitic alphavirus; Authors - Saravanan Raju

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sampath (Verified Customer) (03-11-2020)

Pcdh10 antibody worked very well for me. I saw the bands even after 5 sec of exposure at dilution 1:1000 o/n incubation.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Brain tissue

PCDH10 Antibody Western Blot, validation (1:1000 dilution) in Brain tissue (Cat no:21859-1-AP)

FH

Aurelie (Verified Customer) (06-13-2019)

In tissue, there is some background but the antibody give a correct signal. I also try in human primary fibroblasts, the background is lower and the band at 130kd observed here , is not present.- dilution 1/1500 should be enough

• Applications: Western Blot,
• Primary Antibody Dilution: 1/800
• Cell Tissue Type: Mouse Cortex

PCDH10 Antibody Western Blot, validation (1/800 dilution) in Mouse Cortex (Cat no:21859-1-AP)