Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue, mouse liver tissue |
| Positive IHC detected in | human breast cancer tissue, mouse kidney tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14892-1-AP targets PCK1/2 in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6681 Product name: Recombinant human PCK2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 292-640 aa of BC001454 Sequence: AGKKRYVAAAFPSACGKTNLAMMRPALPGWKVECVGDDIAWMRFDSEGRLRAINPENGFFGVAPGTSATTNPNAMATIQSNTIFTNVAETSDGGVYWEGIDQPLPPGVTVTSWLGKPWKPGDKEPCAHPNSRFCAPARQCPIMDPAWEAPEGVPIDAIIFGGRRPKGVPLVYEAFNWRHGVFVGSAMRSESTAAAEHKGKIIMHDPFAMRPFFGYNFGHYLEHWLSMEGRKGAQLPRIFHVNWFRRDEAGHFLWPGFGENARVLDWICRRLEGEDSARETPIGLVPKEGALDLSGLRAIDTTQLFSLPKDFWEQEVRDIRSYLTEQVNQDLPKEVLAELEALERRVHKM Predict reactive species |
| Full Name | phosphoenolpyruvate carboxykinase 2 (mitochondrial) |
| Calculated Molecular Weight | 71 kDa |
| Observed Molecular Weight | 66-71 kDa |
| GenBank Accession Number | BC001454 |
| Gene Symbol | PCK2 |
| Gene ID (NCBI) | 5106 |
| RRID | AB_2160044 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16822 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PCK2(phosphoenolpyruvate carboxykinase [GTP], mitochondrial) is also named as PEPCK2, PEPCK-M and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyses the irreversible conversion of oxaloacetate to phosphoenolpyruvate, an important initial step in hepatic gluconeogenesis.Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD). It has 2 isoforms produced by alternative splicing with the molecular weight of 71 kDa and 48 kDa. This antibody has weak cross-reactivity with PCK1 protein due to high sequence homology between PCK1 and PCK2.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PCK1/2 antibody 14892-1-AP | Download protocol |
| IF protocol for PCK1/2 antibody 14892-1-AP | Download protocol |
| IHC protocol for PCK1/2 antibody 14892-1-AP | Download protocol |
| WB protocol for PCK1/2 antibody 14892-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PCK1/2 Polyclonal antibody (Cat# 14892-1-AP) has 49 citations. Publications appear in journals including Advanced Science, Science Advances, Cell Death & Disease, EBioMedicine, Diabetologia, Journal of Biological Chemistry, EMBO Reports, Cell Signaling, and Journal of Immunology, among others. Research fields span metabolic reprogramming and gluconeogenesis, diabetes and insulin resistance, hepatic steatosis and NAFLD, cancer biology (lung, gastric, glioblastoma, medulloblastoma), ferroptosis, mitochondrial biology, and stroke/neurological injury.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) The Lactate-Primed KAT8‒PCK2 Axis Exacerbates Hepatic Ferroptosis During Ischemia/Reperfusion Injury by Reprogramming OXSM-Dependent Mitochondrial Fatty Acid Synthesis
| ||
Sci Adv Atf3-mediated metabolic reprogramming in hepatic macrophage orchestrates metabolic dysfunction-associated steatohepatitis | ||
Cell Death Dis CHCHD2 and CHCHD10 regulate mitochondrial dynamics and integrated stress response. | ||
Cell Death Dis 18F-FDG-PET/CT-negative gastric cancer employs glutamine-based gluconeogenesis and fatty acid oxidation to support tumor growth. | ||
EBioMedicine Homozygous variant in translocase of outer mitochondrial membrane 7 leads to metabolic reprogramming and microcephalic osteodysplastic dwarfism with moyamoya disease | ||
Diabetologia Targeting BCAA metabolism to potentiate metformin's therapeutic efficacy in the treatment of diabetes in mice |
Reviews
What customers say
The single review for this product is a 5-star rating from an immunofluorescence experiment performed at a 1:200 dilution on transgenic mouse kidney tissue (TRACK). The reviewer reported successful detection of PCK2 (red) in proximal tubules where HIF1A signaling is activated, indicating the antibody performed well for its intended application in visualizing proximal tubule markers in a kidney-specific genetic background.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Kristian (Verified Customer) (06-07-2021) | PCK2 (red) is detected in proximale tubules where HIF1A-signaling is activated.
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