PCK2 Polyclonal antibody

PCK2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

29

Reviews (1)

Cat no : 14892-1-AP

Synonyms

Phosphoenolpyruvate carboxykinase 2, mitochondrial, PEPCK-M, PEPCK2, mtPCK2, EC:4.1.1.32

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Validation Data Gallery

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  WB analysis of mouse brain using 14892-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14892-1-AP (PCK2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 14892-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14892-1-AP (PCK2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 14892-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14892-1-AP (PCK2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 14892-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14892-1-AP (PCK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14892-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14892-1-AP (PCK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 14892-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14892-1-AP (PCK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 14892-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14892-1-AP (PCK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14892-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14892-1-AP (PCK2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 14892-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14892-1-AP (PCK2 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of MCF-7 using 14892-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 14892-1-AP (PCK2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue, mouse liver tissue
• Positive IHC detected in: human breast cancer tissue, mouse kidney tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 24 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

14892-1-AP targets PCK2 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PCK2 fusion protein Ag6681
• Full Name: phosphoenolpyruvate carboxykinase 2 (mitochondrial)
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 66-71 kDa
• GenBank Accession Number: BC001454
• Gene Symbol: PCK2
• Gene ID (NCBI): 5106
• RRID: AB_2160044
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PCK2(phosphoenolpyruvate carboxykinase [GTP], mitochondrial) is also named as PEPCK2, PEPCK-M and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyses the irreversible conversion of oxaloacetate to phosphoenolpyruvate, an important initial step in hepatic gluconeogenesis.Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD). It has 2 isoforms produced by alternative splicing with the molecular weight of 71 kDa and 48 kDa.

Publications

Species	Application	Title
human	WB	Sci Rep; TCF7L2 involvement in estradiol- and progesterone-modulated islet and hepatic glucose homeostasis.; Authors - Fengqin Dong
mouse	WB	Mol Nutr Food Res; Douchi Peptides Vy and Sfllr Improve Glucose Homeostasis and Gut Dysbacteriosis In High-Fat Diet-Induced Insulin Resistant Mice; Authors - Songfeng Yu
mouse	WB	J Nat Prod; Effect of 1-Deoxynojirimycin Isolated from Mulberry Leaves on Glucose Metabolism and Gut Microbiota in a Streptozotocin-Induced Diabetic Mouse Model; Authors - Teng-Gen Hu
rat	IF	Mol Neurobiol; Phosphoenolpyruvate Carboxykinase (PCK) in the Brain Gluconeogenic Pathway Contributes to Oxidative and Lactic Injury After Stroke.; Authors - Xiaokun Geng
rat	WB	Front Pharmacol; Bicyclol Regulates Hepatic Gluconeogenesis in Rats with Type 2 Diabetes and Non-alcoholic Fatty Liver Disease by Inhibiting Inflammation.; Authors - Hongxue Li
mouse	WB	Food Funct; Polysaccharides from Armillariella tabescens mycelia ameliorate insulin resistance in type 2 diabetic mice.; Authors - Rui Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kristian (Verified Customer) (06-07-2021)

PCK2 (red) is detected in proximale tubules where HIF1A-signaling is activated.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Transgenic mouse kidney (TRACK)

PCK2 Antibody Immunofluorescence validation (1:200 dilution) in Transgenic mouse kidney (TRACK) (Cat no:14892-1-AP)