CoraLite®594-conjugated PCNA Polyclonal antibody

• Featured Product
• KD/KO Validated

PCNA Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-10205

Synonyms

Cyclin, PCNA

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Validation Data Gallery

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  IF Staining of human breast cancer using CL594-10205

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 PCNA antibody (CL594-10205) at dilution of 1:200.

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  IF Staining of mouse testis using CL594-10205

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 PCNA antibody (CL594-10205) at dilution of 1:200.

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  IF Staining of human liver cancer using CL594-10205

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 PCNA antibody (CL594-10205) at dilution of 1:200.

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  FC experiment of Jurkat using CL594-10205

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PCNA (CL594-10205) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of HeLa using CL594-10205

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PCNA (CL594-10205) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of HeLa using CL594-10205

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human PCNA (CL594-10205) (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF detected in: human breast cancer tissue, human liver cancer tissue, mouse testis tissue
• Positive FC detected in: Jurkat cells, HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Product Information

CL594-10205 targets PCNA in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PCNA fusion protein Ag0277
• Full Name: proliferating cell nuclear antigen
• Calculated molecular weight: 29 kDa/31 kDa
• Observed molecular weight: 36-38 kDa
• GenBank accession number: BC000491
• Gene symbol: PCNA
• Gene ID (NCBI): 5111
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. This antibody is a rabbit polyclonal antibody raised against an internal region of human PCNA. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).

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